Department of Geriatric Endocrinology, Chinese PLA General Hospital, Beijing, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(14):3239-3246.
This study aimed to investigate the expression and clinical significances of long noncoding RNA-ATB (lncRNA-ATB) in papillary thyroid cancer (PTC), and to explore the roles of lncRNA-ATB in PTC cell proliferation and migration.
The expression of lncRNA-ATB in 64 PTC tissues and paired adjacent noncancerous thyroid tissues was measured. The association between lncRNA-ATB expression and clinicopathological characteristics was analyzed by Pearson X2. The diagnostic value of lncRNA-ATB was evaluated by receiver operating characteristic curve (ROC) analyses. The effects of lncRNA-ATB on PTC cell proliferation were evaluated by Cell Counting Kit-8 assays and Ethynyl deoxyuridine incorporation assays. The effects of lncRNA-ATB on PTC cell migration were evaluated by transwell assays.
LncRNA-ATB is upregulated in PTC tissues compared with noncancerous tissues. LncRNA-ATB is also increased in PTC cell lines compared with normal thyroid follicular epithelial cell line. High-expression of lncRNA-ATB is associated with large tumor size and lymph node metastasis. ROC analyses revealed that lncRNA-ATB could sensitively discriminate PTCs from noncancerous tissues, as well as discriminating PTCs with lymph node metastasis from those without lymph node metastasis. Functional experiments showed that depletion of lncRNA-ATB significantly inhibits PTC cell proliferation and migration.
LncRNA-ATB is upregulated and functions as an oncogene in PTC. Furthermore, lncRNA-ATB may serve as a diagnostic biomarker and therapeutic target for PTC.
本研究旨在探讨长链非编码 RNA-ATB（lncRNA-ATB）在甲状腺乳头状癌（PTC）中的表达及其临床意义，并探讨 lncRNA-ATB 在 PTC 细胞增殖和迁移中的作用。
检测 64 例 PTC 组织及其配对的癌旁正常甲状腺组织中 lncRNA-ATB 的表达。采用 Pearson X2 分析 lncRNA-ATB 表达与临床病理特征的关系。通过受试者工作特征曲线（ROC）分析评估 lncRNA-ATB 的诊断价值。采用细胞计数试剂盒-8（CCK-8）检测和乙啶核苷掺入实验评估 lncRNA-ATB 对 PTC 细胞增殖的影响。通过 Transwell 实验评估 lncRNA-ATB 对 PTC 细胞迁移的影响。
与癌旁正常组织相比，lncRNA-ATB 在 PTC 组织中上调。与正常甲状腺滤泡上皮细胞系相比，lncRNA-ATB 在 PTC 细胞系中也上调。lncRNA-ATB 的高表达与肿瘤大小较大和淋巴结转移有关。ROC 分析表明，lncRNA-ATB 能够敏感地区分 PTC 与非癌组织，以及区分有淋巴结转移的 PTC 与无淋巴结转移的 PTC。功能实验表明，lncRNA-ATB 的耗竭显著抑制 PTC 细胞的增殖和迁移。
lncRNA-ATB 在 PTC 中上调并发挥癌基因作用。此外，lncRNA-ATB 可能作为 PTC 的诊断生物标志物和治疗靶点。
