Lan Xiabin, Sun Wei, Dong Wenwu, Wang Zhihong, Zhang Ting, He Liang, Zhang Hao
Department of Head and Neck Surgery, Zhejiang Cancer Hospital, No.1, Banshandong Road, Gongshu District, Hangzhou 310022, Zhejiang Province, China; Department of Thyroid Surgery, The First Affiliated Hospital of China Medical University, No. 155, Nanjing Bei Street, Heping District, Shenyang 110001, Liaoning Province, China.
Department of Thyroid Surgery, The First Affiliated Hospital of China Medical University, No. 155, Nanjing Bei Street, Heping District, Shenyang 110001, Liaoning Province, China.
Gene. 2018 Mar 10;646:98-105. doi: 10.1016/j.gene.2017.12.051. Epub 2017 Dec 26.
Recent studies have highlighted important roles for long noncoding RNAs (lncRNAs) during the complex process of carcinogenesis. H19 is an example of an lncRNA that can function either as a tumor promoter or a tumor suppressor. Here, we investigated the role of H19 in papillary thyroid carcinoma (PTC). First, we assessed H19 expression levels in human PTC tissues and PTC cell lines using quantitative real-time PCR. We also established H19-overexpressed PTC cell lines with lentiviral vectors to investigate the effects of H19 on the proliferation and migration of PTC cells. Our results suggest that H19 is downregulated in PTC tissues and in PTC cell lines compared to controls. Decreased H19 expression was correlated with lymph node metastasis. H19 overexpression reduced PTC cell proliferation and migration. It also inhibited the expression of tumor necrosis factor receptor 2. These results suggest that H19 inhibits tumorigenesis in PTC and may be utilized as a potential diagnostic tool for PTC.
最近的研究强调了长链非编码RNA（lncRNAs）在复杂的致癌过程中的重要作用。H19是一种lncRNA的例子，它既可以作为肿瘤促进因子，也可以作为肿瘤抑制因子发挥作用。在这里，我们研究了H19在甲状腺乳头状癌（PTC）中的作用。首先，我们使用定量实时PCR评估了H19在人PTC组织和PTC细胞系中的表达水平。我们还使用慢病毒载体建立了H19过表达的PTC细胞系，以研究H19对PTC细胞增殖和迁移的影响。我们的结果表明，与对照组相比，H19在PTC组织和PTC细胞系中表达下调。H19表达降低与淋巴结转移相关。H19过表达减少了PTC细胞的增殖和迁移。它还抑制了肿瘤坏死因子受体2的表达。这些结果表明，H19抑制PTC的肿瘤发生，可能作为PTC的一种潜在诊断工具。
