Department Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(14):3159-3163.
This study was designed to investigate the influence of several polymorphisms in neuronal NOS genes and apolipoprotein AI on the hormone-related osteonecrosis of the femoral head (ONFH) risk.
Peripheral blood mononuclear cell (PBMCs) samples extracted from hormone-related ONFH patients and controls were used to amplify fragments of the apolipoprotein A1 and neuronal NOS exon 7 and intron 4 using specific PCR primers. The products were analyzed by DNA sequencing and mapped to find the genotype distributions.
The proportions of G/G, G/T and T/T on NOS exon 7 in hormone-related ONFH patient group and control group were 68%, 27%, 5% and 81%, 16%, and 3% respectively. The proportions of G/T and T/T in the experimental group were significantly higher than those in the control group (p<0.05). The proportions of b/b, a/b and a/a on NOS intron 4 in hormone-related ONFH patient group and control group were 85%, 13%, 1% and 96%, 5% and 0% respectively. The proportion of a/b in the experimental group was much higher than in the control group. The distribution of A/A, G/A and G/G in the apolipoprotein gene in control and experimental groups were 19%, 33%, 71% and 42%, 20%, 38% respectively. In this case, the experimental group's A/A genotype was significantly higher than the control's genotype.
In our study group, several polymorphisms of the neuronal NOS gene and apolipoprotein A1 genes were significantly associated with hormone-related ONFH. These results suggest that those gene polymorphisms might be involved in the occurrence and development of ONFH.
本研究旨在探讨神经元型一氧化氮合酶基因和载脂蛋白 AI 中的几种多态性对激素相关性股骨头坏死（ONFH）风险的影响。
从激素相关性 ONFH 患者和对照组中提取外周血单核细胞（PBMC）样本，使用特异性 PCR 引物扩增载脂蛋白 A1 和神经元型一氧化氮合酶外显子 7 和内含子 4 的片段。通过 DNA 测序分析产物并进行基因分型。
NOS 外显子 7 中 G/G、G/T 和 T/T 的比例在激素相关性 ONFH 患者组和对照组中分别为 68%、27%和 5%和 81%、16%和 3%。实验组中 G/T 和 T/T 的比例明显高于对照组（p<0.05）。NOS 内含子 4 中 b/b、a/b 和 a/a 的比例在激素相关性 ONFH 患者组和对照组中分别为 85%、13%和 1%和 96%、5%和 0%。实验组中 a/b 的比例明显高于对照组。对照组和实验组中载脂蛋白基因 A/A、G/A 和 G/G 的分布分别为 19%、33%和 71%和 42%、20%和 38%。在这种情况下，实验组的 A/A 基因型明显高于对照组。
在我们的研究组中，神经元型一氧化氮合酶基因和载脂蛋白 A1 基因的几种多态性与激素相关性 ONFH 显著相关。这些结果表明，这些基因多态性可能参与了 ONFH 的发生和发展。
