Universidad Nacional Mayor de San Marcos, Faculty of Medicine. Medicina y Genética Molecular Materno Perinatal-MEGEMAPE Research Group, Lima, Peru. Universidad Nacional Mayor de San Marcos Universidad Nacional Mayor de San Marcos Faculty of Medicine Medicina y Genética Molecular Materno Perinatal-MEGEMAPE Research Group Lima Peru.
Universidad Nacional Mayor de San Marcos, Faculty of Pharmacy and Biochemistry, Lima, Peru. Universidad Nacional Mayor de San Marcos Universidad Nacional Mayor de San Marcos Faculty of Pharmacy and Biochemistry Lima Peru.
Colomb Med (Cali). 2021 Feb 26;52(1):e2014437. doi: 10.25100/cm.v52i1.4437.
Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbi-mortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms.
To determine the association of the vascular endothelial growth factor (VEGF) +936 C/T and +405 G/C, interleukine-6 (IL-6) -174 G/C, IL-1β-511 C/T, Apo A-1-75 G/A, Apo B-100 2488 C/T (Xbal) polymorphisms with preeclampsia in pregnant Peruvian women.
Were included preeclamptic and healthy (control) pregnant women. Maternal blood samples were subjected to DNA extraction, and molecular genetic analysis was conducted using the PCR-RFLP technique and following a specific protocol for each gene. Allele and genotypic frequencies in the cases and controls were compared.
No association was found between the VEGF+936C/T and VEGF+405 polymorphisms and preeclampsia. The frequencies of the GG genotypes and the G allele of the -174 G/C polymorphism in the IL6 gene in preeclamptic and controls showed significant differences, with higher frequencies in cases. For the -511 C/T polymorphism of the IL-1β gene, no significant differences were found in the frequencies of TT genotypes compared with CT+CC. The genotypes and alleles of the Apo-A1-75 G/A and Apo-B100 Xbal variants showed no significant differences between cases and controls.
No association was found between the studied genetic markers and preeclampsia. However, in the -174G/C polymorphism of the gene, significant differences were found mainly in the GG genotype and G allele.
子痫前期是一种多器官疾病，与母婴围产期发病率和死亡率相关。在秘鲁，子痫前期的发病率为 10%，占孕产妇死亡的 22%。基因组和遗传流行病学研究已经发现子痫前期与遗传多态性之间存在关联。
确定血管内皮生长因子（VEGF）+936 C/T 和 +405 G/C、白细胞介素-6（IL-6）-174 G/C、IL-1β-511 C/T、载脂蛋白 A-1-75 G/A、载脂蛋白 B-100 2488 C/T（Xbal）多态性与秘鲁孕妇子痫前期的相关性。
纳入子痫前期患者和健康（对照）孕妇。采集孕妇血液样本进行 DNA 提取，采用 PCR-RFLP 技术和针对每个基因的特定方案进行分子遗传学分析。比较病例组和对照组的等位基因和基因型频率。
VEGF+936C/T 和 VEGF+405 多态性与子痫前期之间未发现相关性。IL6 基因-174G/C 多态性 GG 基因型和 G 等位基因在子痫前期患者和对照组中的频率存在显著差异，病例组中的频率更高。对于 IL-1β 基因-511 C/T 多态性，TT 基因型与 CT+CC 相比，频率无显著差异。Apo-A1-75 G/A 和 Apo-B100 Xbal 变体的基因型和等位基因在病例组和对照组之间无显著差异。
在所研究的遗传标记与子痫前期之间未发现相关性。然而，在基因的-174G/C 多态性中，主要在 GG 基因型和 G 等位基因中发现了显著差异。
