Ding Lu, Li Rong, Sun Rongxin, Zhou Yang, Zhou Yubo, Han Xiaoping, Cui Yong, Wang Wu, Lv Qing, Bai Jingping
a Department of Orthopedics , Fifth Affiliated Hospital, Xinjiang Medical University , Xinjiang , China.
b Department of Orthopedics , Tumor Hospital Affiliated to Xinjiang Medical University , Xinjiang , China.
Cell Cycle. 2017 Aug 18;16(16):1547-1555. doi: 10.1080/15384101.2017.1346760. Epub 2017 Aug 3.
Skp2 (S-phase kinase-associated protein 2) plays an oncogenic role in a variety of human cancers. However, the function of Skp2 in osteosarcoma (OS) is elusive. Therefore, in the current study, we explore whether Skp2 exerts its oncogenic function in OS. The cell growth, apoptosis, invasion and cell cycle were measured in OS cells after Skp2 overexpression. We found that overexpression of Skp2 enhanced cell growth, and inhibited cell apoptosis in OS cells. Moreover, we observed that upregulation of Skp2 accelerated cell cycle progression in OS cells. Furthermore, the ability of migration and invasion was enhanced in Skp2 overexpressing OS cells. Mechanically, our Western blotting data suggested that Skp2 decreased the expression of E-cadherin, Foxo1, p21, and p57, but increased MMP-9 in OS cells. In conclusion, our study demonstrated that Skp2 exhibited an oncogenic function in OS cells, suggesting that inhibition of Skp2 may be a novel approach for the treatment of OS.
Skp2（S期激酶相关蛋白2）在多种人类癌症中发挥致癌作用。然而，Skp2在骨肉瘤（OS）中的功能尚不清楚。因此，在本研究中，我们探讨Skp2是否在OS中发挥其致癌功能。在Skp2过表达后，检测OS细胞中的细胞生长、凋亡、侵袭和细胞周期。我们发现Skp2的过表达增强了OS细胞的生长，并抑制了细胞凋亡。此外，我们观察到Skp2的上调加速了OS细胞的细胞周期进程。此外，Skp2过表达的OS细胞迁移和侵袭能力增强。机制上，我们的蛋白质印迹数据表明，Skp2降低了OS细胞中E-钙黏蛋白、Foxo1、p21和p57的表达，但增加了MMP-9的表达。总之，我们的研究表明Skp2在OS细胞中表现出致癌功能，提示抑制Skp2可能是治疗OS的一种新方法。
