从有不同症状的女性中分离出的阴道白色念珠菌所造成的细胞损伤。

Cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies.

作者信息

Faria Daniella Renata, Sakita Karina Mayumi, Akimoto-Gunther Luciene Setsuko, Kioshima Érika Seki, Svidzinski Terezinha Inez Estivalet, Bonfim-Mendonça Patrícia de Souza

机构信息

Division of Medical Mycology, Teaching and Research Laboratory in Clinical Analyses, Department of Clinical Analysis of State University of Maringá, Paraná, Brazil.

出版信息

J Med Microbiol. 2017 Aug;66(8):1225-1228. doi: 10.1099/jmm.0.000547. Epub 2017 Aug 10.

DOI:10.1099/jmm.0.000547

PMID:28771140

Abstract

The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and 4 from women with recurrent vulvovaginal candidiasis (RVVC). We evaluated the ability of C. albicans to adhere to human cervical cancer cells (SiHa), the yeast-SiHa cell interactions and cell damage. All of the clinical isolates presented a high adhesion capacity on SiHa cells. However, clinical isolates from symptomatic women (VVC and RVVC) had higher filamentation after contact (24 h) with SiHa cells and a greater capacity to cause cell damage (>80 %). Clinical isolates from symptomatic women had greater potential to invade SiHa cells, suggesting that they are more pathogenic than AS isolates.

摘要

本研究旨在表征由来自不同症状女性的阴道白色念珠菌分离株所引起的细胞损伤。对来自阴道样本的12株白色念珠菌临床分离株进行了评估：4株来自无症状女性（AS），4株来自患有单次外阴阴道念珠菌病（VVC）的女性，4株来自患有复发性外阴阴道念珠菌病（RVVC）的女性。我们评估了白色念珠菌黏附人宫颈癌细胞（SiHa）的能力、酵母 - SiHa细胞相互作用以及细胞损伤情况。所有临床分离株在SiHa细胞上均表现出高黏附能力。然而，来自有症状女性（VVC和RVVC）的临床分离株在与SiHa细胞接触（24小时）后具有更高的丝状化程度，并且具有更大的导致细胞损伤的能力（>80%）。来自有症状女性的临床分离株具有更大的侵袭SiHa细胞的潜力，这表明它们比AS分离株更具致病性。