Ratti Bianca Altrão, Godoy Janine Silva Ribeiro, de Souza Bonfim Mendonça Patrícia, Bidóia Danielle Lazarin, Nakamura Tânia Ueda, Nakamura Celso Vataru, Lopes Consolaro Marcia Edilaine, Estivalet Svidzinski Terezinha Inez, de Oliveira Silva Sueli
Programa de Pós-graduação em Biociências Aplicadas á Farmácia, Universidade Estadual de Maringá, Av. Colombo, 5.790, CEP 87020-900 Maringá, PR, Brazil.
Programa de Pós-graduação em Ciências da Saúde, Universidade Estadual de Maringá, Av. Colombo, 5.790, CEP 87020-900 Maringá, PR, Brazil.
Cell Immunol. 2015 Jan;293(1):22-9. doi: 10.1016/j.cellimm.2014.11.004. Epub 2014 Dec 5.
Vulvovaginal candidiasis (VVC) is characterized by an infection of the vulva and vagina, mainly caused by Candida albicans, a commensal microorganism that inhabits the vaginal, digestive, and respiratory mucosae. Vulvovaginal candidiasis affects approximately 75% of women, and 5% develop the recurrent form (RVVC). The aim of the present study was to evaluate whether neutrophils microbicidal response is triggered when activated with RVVC isolates caused by C. albicans. Our results showed that RVVC isolates induced neutrophil migration but significantly decrease the microbicidal activity of neutrophils, compared with VVC and ASS isolates. The microbicidal activity of neutrophils is highly dependent on the production of reactive oxygen species/reactive nitrogen species (ROS/RNS). However, this isolate induced detoxification of ROS/RNS produced by neutrophils, reflected by the high level of thiol groups and by the oxygen consumption. Therefore, RVVC isolates induced biochemical changes in the inflammatory response triggered by neutrophils, and these effects were mainly related to the detoxification of ROS/RNS through the thioredoxin reductase (TR), a key antioxidant enzyme in fungi. This might be one of the resistance mechanisms triggered by RVVC caused by C. albicans.
外阴阴道念珠菌病（VVC）的特征是外阴和阴道感染，主要由白色念珠菌引起，白色念珠菌是一种存在于阴道、消化道和呼吸道黏膜的共生微生物。外阴阴道念珠菌病影响约75%的女性，其中5%会发展为复发性形式（RVVC）。本研究的目的是评估用白色念珠菌引起的RVVC分离株激活中性粒细胞时，是否会触发其杀菌反应。我们的结果表明，与VVC和ASS分离株相比，RVVC分离株诱导中性粒细胞迁移，但显著降低了中性粒细胞的杀菌活性。中性粒细胞的杀菌活性高度依赖于活性氧/活性氮（ROS/RNS）的产生。然而，这种分离株诱导了中性粒细胞产生的ROS/RNS的解毒作用，这通过高水平的巯基和耗氧量得以体现。因此，RVVC分离株在中性粒细胞触发的炎症反应中诱导了生化变化，这些影响主要与通过硫氧还蛋白还原酶（TR）对ROS/RNS的解毒作用有关，硫氧还蛋白还原酶是真菌中的一种关键抗氧化酶。这可能是白色念珠菌引起的RVVC触发的耐药机制之一。
