Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Aachen, Germany.
Caries Res. 2017;51(5):451-459. doi: 10.1159/000477215. Epub 2017 Aug 4.
The application of a self-assembling peptide on noncavitated caries lesions is supposed to be a feasible approach to facilitate remineralization and mask their unfavorable appearance. However, demineralizing conditions are common in the oral environment, so the aim of this pH-cycling study was to compare recommended and novel treatment methods regarding their ability to hamper demineralization and as a consequence mask artificial enamel caries lesions. Artificial caries lesions were prepared in bovine enamel and randomly allocated to 11 groups (n = 22). Treatments before pH-cycling were as follows: the application of a self-assembling peptide (Curodont™ Repair [C]), a low-viscosity resin (Icon® [I]), 2 fluoride solutions (10,000 ppm F-: Elmex fluid [E] and 43,350 ppm F-: Tiefenfluorid® [T]), and no intervention (N). During pH-cycling (28 days, 6 × 60 min demineralization/day) half of the specimens in each group were brushed (10 s; 2 ×/day) with either fluoride-free (named e.g., C0) or NaF (1,100 ppm F-; e.g., C1) dentifrice slurry. In another subgroup specimens were pH-cycled but not brushed (NNB). Differences in integrated mineral loss (ΔΔZ), lesion depth (ΔLD), and colorimetric values (ΔΔE) were calculated between values after pre-demineralization, surface treatment, and pH-cycling. Specimens of C0, C1, NNB, N0, N1, T0, and E0 showed significantly increased ΔZ and LD values after pH-cycling (p ≤ 0.003; paired t test). C0, C1, NNB, and N0 showed significantly higher changes in ΔΔZ than E1, I0, I1, and T1 (p < 0.001; ANOVA). Significantly reduced colorimetric values could only be observed for I1, I0, E1, and E0 after treatment and after pH-cycling (p ≤ 0.027; paired t test). In conclusion, under the conditions chosen only the application of a low-viscosity resin could mask caries lesions significantly, whereas self-assembling peptides could neither inhibit lesion progression nor mask the lesions considerably.
应用自组装肽于非窝沟龋损被认为是一种促进再矿化和掩盖其不利外观的可行方法。然而，脱矿环境在口腔环境中很常见，因此，本 pH 循环研究的目的是比较推荐和新型治疗方法在抑制脱矿作用方面的能力，以及因此掩盖人工釉质龋损的能力。人工龋损在牛牙釉质上制备，并随机分配到 11 个组（n = 22）。pH 循环前的处理如下：应用自组装肽（Curodont™ Repair [C]）、低粘度树脂（Icon® [I]）、2 种氟化物溶液（10,000 ppm F-：Elmex 液 [E]和 43,350 ppm F-：Tiefenfluorid® [T]）和无干预（N）。在 pH 循环（28 天，每天 6 次×60 分钟脱矿化）期间，每组的一半标本用无氟（例如，C0）或 NaF（1,100 ppm F-；例如，C1）牙膏糊剂刷牙（10 s；每天 2 次）。在另一个亚组中，标本进行 pH 循环但不刷牙（NNB）。在预脱矿、表面处理和 pH 循环后计算整合矿物损失（ΔΔZ）、病变深度（ΔLD）和比色值（ΔΔE）之间的差异。在 pH 循环后，C0、C1、NNB、N0、N1、T0 和 E0 的 ΔZ 和 LD 值均显著增加（p ≤ 0.003；配对 t 检验）。与 E1、I0、I1 和 T1 相比，C0、C1、NNB 和 N0 的 ΔΔZ 变化显著更高（p < 0.001；方差分析）。仅在处理后和 pH 循环后才能观察到 I1、I0、E1 和 E0 的比色值显著降低（p ≤ 0.027；配对 t 检验）。总之，在所选择的条件下，只有应用低粘度树脂才能显著掩盖龋损，而自组装肽既不能抑制病变进展，也不能显著掩盖病变。
