Ge Yang, Ren Biao, Zhou Xuedong, Xu Hockin H K, Wang Suping, Li Mingyun, Weir Michael D, Feng Mingye, Cheng Lei
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610000, China.
Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610000, China.
Materials (Basel). 2017 Jan 3;10(1):26. doi: 10.3390/ma10010026.
The mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. , , and were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications.
首次研究了一种含有聚（酰胺胺）树枝状大分子（PAMAM）和甲基丙烯酸二甲基氨基十二烷基酯（DMADDM）的新型抗龋胶粘剂的力学性能和抗龋效果。对新型抗龋胶粘剂进行了微拉伸粘结强度和表面电荷密度的测量。选择变形链球菌、嗜酸乳杆菌和血链球菌形成三种细菌生物膜。进行乳酸测定、MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）测定、胞外多糖染色和活/死染色，以研究该胶粘剂的抗生物膜效果。采用TaqMan实时聚合酶链反应研究不同组三种细菌生物膜的比例变化。使用扫描电子显微镜（SEM）观察PAMAM和DMADDM的再矿化效果。结果表明，在胶粘剂中加入PAMAM和DMADDM对牙本质粘结强度没有不利影响。1%PAMAM和5%DMADDM胶粘剂组表现出抗生物膜特性，并形成了更健康的生物膜，诱发龋齿的可能性更低。1%PAMAM和5%DMADDM溶液的组合在牙本质上保持了再矿化能力，与单独使用1%PAMAM相似。总之，含有PAMAM和DMADDM的胶粘剂具有很强的抗菌性能和生物再矿化能力，在抗龋临床应用中具有广阔前景。
