Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
J Dent. 2013 Apr;41(4):345-55. doi: 10.1016/j.jdent.2013.01.004. Epub 2013 Jan 23.
The main reason for restoration failure is secondary caries caused by biofilm acids. Replacing the failed restorations accounts for 50-70% of all operative work. The objectives of this study were to incorporate a new quaternary ammonium monomer (dimethylaminododecyl methacrylate, DMADDM) and nanoparticles of silver (NAg) into a primer and an adhesive, and to investigate their effects on antibacterial and dentin bonding properties.
Scotchbond Multi-Purpose (SBMP) served as control. DMADDM was synthesized and incorporated with NAg into primer/adhesive. A dental plaque microcosm biofilm model with human saliva was used to investigate metabolic activity, colony-forming units (CFU), and lactic acid. Dentin shear bond strengths were measured.
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the new DMADDM were orders of magnitude lower than those of a previous quaternary ammonium dimethacrylate (QADM). Uncured primer with DMADDM had much larger inhibition zones than QADM (p<0.05). Cured primer/adhesive with DMADDM-NAg greatly reduced biofilm metabolic activity (p<0.05). Combining DMADDM with NAg in primer/adhesive resulted in less CFU than DMADDM alone (p<0.05). Lactic acid production by biofilms was reduced by 20-fold via DMADDM-NAg, compared to control. Incorporation of DMADDM and NAg into primer/adhesive did not adversely affect dentin bond strength.
A new antibacterial monomer DMADDM was synthesized and incorporated into primer/adhesive for the first time. The bonding agents are promising to combat residual bacteria in tooth cavity and invading bacteria at tooth-restoration margins to inhibit caries. DMADDM and NAg are promising for use into a wide range of dental adhesive systems and restoratives.
修复失败的主要原因是生物膜酸引起的继发龋。替换失败的修复体占所有手术工作的 50-70%。本研究的目的是将一种新的季铵单体（二甲氨基十二烷基甲基丙烯酸酯，DMADDM）和纳米银（NAg）纳入底漆和胶粘剂中，并研究它们对抗菌和牙本质粘结性能的影响。
Scotchbond Multi-Purpose（SBMP）作为对照。DMADDM 被合成并与 NAg 一起掺入底漆/胶粘剂中。使用含有人唾液的牙菌斑微宇宙生物膜模型来研究代谢活性、菌落形成单位（CFU）和乳酸。测量牙本质剪切粘结强度。
新 DMADDM 的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）均比以前的季铵基二甲基丙烯酸酯（QADM）低几个数量级。未固化的含有 DMADDM 的底漆具有比 QADM 更大的抑菌圈（p<0.05）。用 DMADDM 固化的底漆/胶粘剂大大降低了生物膜的代谢活性（p<0.05）。将 DMADDM 与 NAg 结合在底漆/胶粘剂中会导致 CFU 比单独使用 DMADDM 减少（p<0.05）。与对照相比，DMADDM-NAg 使生物膜产生的乳酸减少了 20 倍。将 DMADDM 和 NAg 掺入底漆/胶粘剂中不会对牙本质粘结强度产生不利影响。
首次合成并将一种新的抗菌单体 DMADDM 纳入底漆/胶粘剂中。这些粘结剂有望对抗牙腔中的残留细菌和牙修复边缘的入侵细菌，以抑制龋齿。DMADDM 和 NAg 有望用于广泛的牙科粘结剂系统和修复体。
