Young E G, Hanson M R, Dierks P M
Nucleic Acids Res. 1986 Oct 24;14(20):7995-8006. doi: 10.1093/nar/14.20.7995.
We have sequenced the Petunia hybrida gene that specifies the proteolipid subunit of the mitochondrial Fo ATP synthase and have used this gene to investigate plant mitochondrial gene transcription. The Petunia atp 9 gene contains a single open-reading frame capable of specifying a 77 amino acid-polypeptide that is homologous to bovine, fungal and maize proteolipid subunits. S1 protection identified 3 transcripts in a ratio of 1:5:100 in the Petunia tissues tested. The transcripts share a common 3' terminus but have 5' termini that map 528, 266, and 121 nucleotides upstream of the translation start site. The 5' terminus of the longest transcript maps to the sequence ATATAGTA, which is nearly identical to the yeast mitochondrial transcription initiation site ATATAAGTA. Primer extension analysis indicates that these two shorter transcripts are not due to splicing. The two shorter transcripts originate at sequences homologous to sites at 5' termini of two pea and maize genes. These consensus sequences may signal processing events other than splicing.
我们已对矮牵牛中指定线粒体F₀ATP合酶蛋白脂质亚基的基因进行了测序，并利用该基因研究植物线粒体基因转录。矮牵牛atp 9基因包含一个单一的开放阅读框，能够指定一个与牛、真菌和玉米蛋白脂质亚基同源的77个氨基酸的多肽。S1核酸酶保护实验在测试的矮牵牛组织中鉴定出3种转录本，其比例为1:5:100。这些转录本共享一个共同的3'末端，但5'末端分别位于翻译起始位点上游528、266和121个核苷酸处。最长转录本的5'末端定位于序列ATATAGTA，该序列与酵母线粒体转录起始位点ATATAAGTA几乎相同。引物延伸分析表明，这两个较短的转录本不是由剪接产生的。这两个较短的转录本起源于与两个豌豆和玉米基因5'末端位点同源的序列。这些共有序列可能标志着除剪接之外的其他加工事件。
