Department of Dermatology, University of Groningen, University Medical Centre Groningen, 9700 RB, Groningen, The Netherlands.
Department of Occupational and Environmental Dermatology, Lund University, Skåne University Hospital, 205 02, Malmö, Sweden.
Contact Dermatitis. 2018 Jan;78(1):7-11. doi: 10.1111/cod.12857. Epub 2017 Aug 4.
During rubber vulcanization, new compounds can be formed.
To report a case of allergic shoe dermatitis in which the search for the allergen ultimately led to the identification of dimethylthiocarbamylbenzothiazole sulfide (DMTBS).
A female presented with eczema on her feet after wearing Sperry Top Sider® canvas sneakers. Patch testing was performed with the European baseline series, additional series, shoe materials, and extracts of shoe materials. Thin-layer chromatography (TLC) was performed for additional patch testing, and high-performance liquid chromatography and gas chromatography-mass spectometry were used for chemical analysis.
Positive reactions were found to thiuram mix (+), tetramethylthiuram monosulfide (TMTM) (+), shoe material (+), and shoe extracts in eth. (++) and acetone (+). The extracts did not contain TMTM or other components of thiuram mix. TLC strips yielded a positive reaction (+) to one spot, whereas chemical analysis gave a negative result. Thereafter, a similar sneaker from another patient with shoe dermatitis was analysed, and DMBTS was identified. New extracts of the shoe of our first patient were then also shown to contain DMTBS. DMTBS as culprit allergen was confirmed by positive patch testing with a dilution series with DMTBS.
DMBTS was identified as the culprit allergen in shoe dermatitis, giving rise to compound allergy. The positive reaction to TMTM was considered to represent cross-reactivity.
在橡胶硫化过程中，可能会形成新的化合物。
报告 1 例因穿着斯佩里托普赛德帆布运动鞋而导致的过敏性鞋接触性皮炎，过敏原的寻找最终导致鉴定出二甲基硫代羰氨苯并噻唑次磺酰胺（DMTBS）。
1 名女性在穿着斯佩里托普赛德帆布运动鞋后足部出现湿疹，对其进行了斑贴试验，包括欧洲基本系列、附加系列、鞋用材料和鞋用材料提取物。进行了薄层色谱（TLC）以进行额外的斑贴试验，同时还进行了高效液相色谱和气相色谱-质谱分析。
对硫代双胍混合物（+）、四甲基秋兰姆单硫化物（TMTM）（+）、鞋用材料（+）和鞋用材料提取物（乙醇中为（++），丙酮中为（+））的反应呈阳性。提取物中未检出 TMTM 或硫代双胍混合物的其他成分。TLC 条带显示 1 个阳性反应点（+），而化学分析结果为阴性。此后，对另 1 例患有鞋接触性皮炎的患者的类似运动鞋进行了分析，并鉴定出 DMBTS。然后还对我们第 1 例患者的鞋用新提取物进行了分析，结果也显示含有 DMTBS。DMTBS 作为变应原，通过用 DMTBS 进行稀释系列的斑贴试验得到了确认。
DMBTS 被鉴定为鞋接触性皮炎的变应原，引起复合变应性。对 TMTM 的阳性反应被认为是交叉反应。
