ACBD3作为一种支架蛋白，用于组织高尔基体堆叠蛋白以及一种Rab33b鸟苷酸激活蛋白。
ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP.
作者信息
Yue Xihua, Bao Mengjing, Christiano Romain, Li Siyang, Mei Jia, Zhu Lianhui, Mao Feifei, Yue Qiang, Zhang Panpan, Jing Shuaiyang, Rothman James E, Qian Yi, Lee Intaek
机构信息
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, China.
Department of Genetics and Complex Diseases, School of Public Health, Harvard medical school, Boston, MA, USA.
出版信息
FEBS Lett. 2017 Sep;591(18):2793-2802. doi: 10.1002/1873-3468.12780. Epub 2017 Aug 21.
Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing 3 (ACBD3) is likely to be a novel binding partner of Golgin45. ACBD3 interacts with Golgin45 via its GOLD domain, while its co-expression significantly increases Golgin45 targeting to the Golgi. Furthermore, ACBD3 recruits TBC1D22, a Rab33b GTPase activating protein (GAP), to a large multi-protein complex containing Golgin45 and GRASP55. These results suggest that ACBD3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b-GAP at the medial-Golgi.
高尔基体蛋白45（Golgin45）在高尔基体堆叠组装中发挥重要作用，已知它在内侧高尔基体潴泡中与高尔基体堆叠蛋白GRASP55和Rab2结合。在本研究中，我们试图用蛋白质组学方法进一步表征潴泡黏附复合物。我们在此报告，含酰基辅酶A结合结构域3（ACBD3）可能是Golgin45的一个新的结合伙伴。ACBD3通过其GOLD结构域与Golgin45相互作用，而其共表达显著增加Golgin45靶向高尔基体。此外，ACBD3将Rab33b GTP酶激活蛋白（GAP）TBC1D22招募到一个包含Golgin45和GRASP55的大型多蛋白复合物中。这些结果表明，ACBD3可能提供一个支架来在内侧高尔基体中组织高尔基体堆叠蛋白和一个Rab33b-GAP。
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