Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:47-53. doi: 10.1016/j.colsurfb.2017.07.076. Epub 2017 Jul 27.
In this work, a poly-orthophenylene diamine substrate is decorated with gold nanoparticles (GNPs) and then with single-stranded DNA (ss-DNA) aptamer immobilized on the surface of a pencil graphite electrode (PGE) for the detection and determination of plasma insulin. In this procedure, a polymer layer is formed on the surface of the graphite electrode using anodic oxidation of orthophenylene diamine monomers. The parameters affecting the biosensor sensitivity including the potential applied to form the polymer layer, incubation time, concentration of immobilized ss-DNA, and the presence of MgCl (as salt) are evaluated. Electrochemical impedance spectroscopy and cyclic voltammetry are then used to characterize the biosensor. The biosensor is employed under optimal conditions to obtain figures of merit, which reveal a linear range and a limit of detection of 1.0-1000.0nmolL and 0.27nmolL, respectively. Finally, the aptasensor is employed for the determination of insulin in real plasma and urine samples to verify its performance and effectiveness.
在这项工作中，聚邻苯二胺基底上修饰了金纳米粒子（GNPs），然后将固定在铅笔石墨电极（PGE）表面的单链 DNA（ss-DNA）适体用于检测和测定血浆胰岛素。在该过程中，使用邻苯二胺单体的阳极氧化在石墨电极表面形成聚合物层。评估了影响生物传感器灵敏度的参数，包括形成聚合物层的施加电位、孵育时间、固定 ss-DNA 的浓度以及 MgCl（作为盐）的存在。然后使用电化学阻抗谱和循环伏安法对生物传感器进行了表征。在最佳条件下使用生物传感器获得了衡量标准，显示出线性范围和检测限分别为 1.0-1000.0nmolL 和 0.27nmolL。最后，将适体传感器用于测定真实血浆和尿液样品中的胰岛素，以验证其性能和有效性。
