Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:54-61. doi: 10.1016/j.colsurfb.2017.07.059. Epub 2017 Jul 25.
DNA vaccination with microneedles (MNs) into the skin represents a potential therapeutic approach for the clinical treatment of viral diseases as well as for intradermal genetic immunization. In this study, we investigated a DNA vaccination against the severe fever with thrombocytopenia syndrome virus (SFTSV) delivered by nano-patterned microneedles (nMNs) to improve the efficiency compared to a conventional MN vaccination. Because DNA vaccinations delivered by coated MNs have major disadvantages such as a poor coating efficiency and immunogenicity, additional excipients are necessary. Therefore, we developed nMNs to improve the affinity of stainless steel for plasmid DNA vaccinations. The results show that the nMNs have an improved DNA vaccine loading capacity because their surfaces have an increased hydrophilicity from the high surface/volume ratio. The cytocompatibility analysis also showed a higher cell proliferation when using the nMNs. Finally, the in vivo experiments with balb/c mice vaccinated with the SFTSV DNA vaccine-coated nMNs generated a higher level of cellular immune responses than that of the unmodified MNs.
微针（MNs）接种 DNA 疫苗进入皮肤代表了一种治疗病毒疾病的潜在治疗方法，以及皮内基因免疫。在这项研究中，我们研究了一种针对严重发热伴血小板减少综合征病毒（SFTSV）的 DNA 疫苗接种，通过纳米图案化微针（nMNs）接种来提高效率，与传统的 MN 疫苗接种相比。由于涂覆 MNs 递送的 DNA 疫苗具有较差的涂覆效率和免疫原性等主要缺点，因此需要额外的赋形剂。因此，我们开发了 nMNs 以提高不锈钢对质粒 DNA 疫苗接种的亲和力。结果表明，nMNs 具有改进的 DNA 疫苗负载能力，因为它们的表面具有高表面积/体积比带来的更高的亲水性。细胞相容性分析还表明，使用 nMNs 时细胞增殖更高。最后，用 SFTSV DNA 疫苗涂覆 nMNs 接种的 balb/c 小鼠的体内实验产生了比未修饰的 MNs 更高水平的细胞免疫反应。
