Sato S, Kabeya H, Negishi A, Tsujimoto H, Nishigaki K, Endo Y, Maruyama S
Laboratory of Veterinary Public Health, Department of Veterinary Medicine,College of Bioresource Sciences, Nihon University,1866 Kameino, Fujisawa, Kanagawa Prefecture, 252-0880,Japan.
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences, The University of Tokyo,1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657,Japan.
Epidemiol Infect. 2017 Oct;145(13):2694-2700. doi: 10.1017/S0950268817001601. Epub 2017 Aug 7.
Cats are known to be the main reservoir for Bartonella henselae and Bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. In the present study, we investigated the prevalence of the two Bartonella species on 1754 cat bloods collected from all prefectures in Japan during 2007-2008 by a nested-polymerase chain reaction (PCR) targeting the 16S-23S rRNA internal transcribed spacer region. Overall, Bartonella DNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected with B. henselae mono-infection, 33·8% (27/80) with B. clarridgeiae mono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) of Bartonella infection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P < 0·001). Statistical analysis of the cats examined suggested a significant association between Bartonella infection and FeLV infection (OR = 1·9; 95% CI = 1·1-3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0-2·6).
猫是汉赛巴尔通体和克拉氏巴尔通体的主要宿主，这两种细菌是人类“猫抓病”的病原体。在本研究中，我们通过针对16S - 23S rRNA内部转录间隔区的巢式聚合酶链反应（PCR），调查了2007 - 2008年期间从日本所有县采集的1754份猫血样中这两种巴尔通体的流行情况。总体而言，在所检测的猫中，4.6%（80/1754）检测到巴尔通体DNA。巢式PCR结果显示，48.8%（39/80）的阳性猫感染了汉赛巴尔通体单一感染，33.8%（27/80）感染了克拉氏巴尔通体单一感染，17.5%（14/80）同时感染了这两种细菌。日本西部巴尔通体感染率（5.9%；65/1103）显著高于东部（2.3%；15/651）（P < 0.001）。对所检测猫的统计分析表明，巴尔通体感染与猫白血病病毒感染之间存在显著关联（OR = 1.9；95% CI = 1.1 - 3.4），但与猫免疫缺陷病毒感染无关（OR = 1.6；95% CI = 1.0 - 2.6）。
