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Structural and functional analyses of the spliceosome requires a multi-disciplinary approach.

作者信息

Ohi Melanie D

机构信息

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, United States; Center for Structural Biology, Vanderbilt University, Nashville, TN 37232, United States.

出版信息

Methods. 2017 Aug 1;125:1-2. doi: 10.1016/j.ymeth.2017.07.022.

Abstract
摘要

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本文引用的文献

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Radical probing of spliceosome assembly.
Methods. 2017 Aug 1;125:16-24. doi: 10.1016/j.ymeth.2017.06.030. Epub 2017 Jun 29.
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Studying structure and function of spliceosomal helicases.
Methods. 2017 Aug 1;125:63-69. doi: 10.1016/j.ymeth.2017.06.028. Epub 2017 Jun 29.
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Reversibly constraining spliceosome-substrate complexes by engineering disulfide crosslinks.
Methods. 2017 Aug 1;125:25-35. doi: 10.1016/j.ymeth.2017.06.022. Epub 2017 Jun 23.
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Structure determination of group II introns.
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Cryo-EM: beyond the microscope.
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The effects of structure on pre-mRNA processing and stability.
Methods. 2017 Aug 1;125:36-44. doi: 10.1016/j.ymeth.2017.06.001. Epub 2017 Jun 6.
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Methodologies for studying the spliceosome's RNA dynamics with single-molecule FRET.
Methods. 2017 Aug 1;125:45-54. doi: 10.1016/j.ymeth.2017.05.011. Epub 2017 May 18.
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Understanding pre-mRNA splicing through crystallography.
Methods. 2017 Aug 1;125:55-62. doi: 10.1016/j.ymeth.2017.04.023. Epub 2017 May 12.
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Structural studies of the endogenous spliceosome - The supraspliceosome.
Methods. 2017 Aug 1;125:70-83. doi: 10.1016/j.ymeth.2017.04.005. Epub 2017 Apr 13.
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A close-up look at the spliceosome, at last.
Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4288-4293. doi: 10.1073/pnas.1700390114. Epub 2017 Apr 13.

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