Cao Wen-Rui, Guo Ling-Yun, Du Zong-Jun, Das Anindita, Saren Gaowa, Jiang Ming-Yu, Dunlap Christopher A, Rooney Alejandro P, Yu Xin-Ke, Li Tie-Gang
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2672-2678. doi: 10.1099/ijsem.0.002006. Epub 2017 Aug 8.
A Gram-stain-positive, aerobic, motile, endospore-forming bacterium, designated strain J15A17T, was isolated from sediment of the South China Sea. The strain was oxidase-positive and catalase-negative. Optimal growth occurred at 33 °C, pH 7.5 and in the presence of 3 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (92.8 %) to Paenibacillus puldeungensis strain CAU 9324T. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family Paenibacillaceae, with the genus Cohnella as the most closely related genus. The DNA G+C content of strain J15A17T was 37.4 mol%. The strain contained MK-7 as the sole respiratory quinone; anteiso-C15 : 0 and iso-C16 : 0 were the major cellular fatty acids; and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and four unidentified phospholipids. The strain displayed the peptidoglycan type A4α l-Lys-d-Asp in the cell wall. Phylogenetic, physiological, biochemical and morphological differences between strain J15A17T and its closest relatives in the genera Cohnella, Fontibacillus and Paenibacillus suggest that strain J15A17T (=KCTC 33759T=MCCC 1H00137T) represents the type strain of a novel species in a new genus within the family Paenibacillaceae, Chengkuizengella sediminis gen. nov. sp. nov.
从中国南海沉积物中分离出一株革兰氏染色阳性、需氧、运动、产芽孢的细菌，命名为J15A17T菌株。该菌株氧化酶阳性，过氧化氢酶阴性。最适生长温度为33 °C，pH值为7.5，在3 %（w/v）NaCl存在的条件下生长。基于16S rRNA基因序列分析，该菌株与Puldeungensis芽孢杆菌CAU 9324T菌株显示出最密切的相似性（92.8 %）。基于16S rRNA基因序列的系统发育分析表明，该分离株在芽孢杆菌科内形成一个单独的分支，与考内拉属关系最为密切。J15A17T菌株的DNA G+C含量为37.4 mol%。该菌株含有MK-7作为唯一的呼吸醌；anteiso-C15 : 0和iso-C16 : 0是主要的细胞脂肪酸；其极性脂质模式由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、糖脂和四种未鉴定的磷脂组成。该菌株在细胞壁中显示出A4α型肽聚糖l-Lys-d-Asp。J15A17T菌株与其在考内拉属、丰芽孢杆菌属和芽孢杆菌属中最密切相关的菌株之间的系统发育、生理、生化和形态学差异表明，J15A17T菌株（=KCTC 33759T=MCCC 1H00137T）代表芽孢杆菌科内一个新属中的一个新物种的模式菌株，即沉积物程奎增氏菌属新属新种。
