Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, PR China.
Key Laboratory of Marine Sedimentology and Environmental Geology, First Institute of Oceanography, SOA, Qingdao 266061, PR China.
Int J Syst Evol Microbiol. 2017 May;67(5):1577-1581. doi: 10.1099/ijsem.0.001767. Epub 2017 May 30.
A Gram-strain-positive, facultatively anaerobic, motile, endospore-forming, slightly halophilic bacterium, designated strain 126C4T, was isolated from sediment from the East China Sea. The strain was catalase-positive and oxidase-negative. Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 3-5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, showed that strain 126C4T was a member of the genus Paraliobacillus, with 16S rRNA gene sequence similarities to Paraliobacillus quinghaiensis YIM-C158T and Paraliobacillus ryukyuensis O15-7T of 96.2 % and 95.3 %, repectively. The DNA G+C content was 39.6 mol%. The strain contained MK-7 as the sole respiratory quinone, anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and its polar lipid pattern comprised diphosphatidylglycerol, phosphatidylethanolamine, three glycolipids and four unknown phospholipids. On the basis of its phylogenetic position, phenotypic traits and chemotaxonomic characteristics, it is suggested that strain 126C4T represents a novel species of the genus Paraliobacillus, for which the name Paraliobacillus sediminis sp. nov. is proposed. The type strain is 126C4T (=KCTC 33762T=MCCC 1H00136T).
一株革兰氏阳性、兼性厌氧、运动、产芽孢、轻度嗜盐的细菌，被命名为 126C4T 菌株，从东海沉积物中分离得到。该菌株过氧化氢酶阳性，氧化酶阴性。最佳生长条件为 28-30°C、pH7.0-7.5 和 3-5%（w/v）NaCl。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 126C4T 是 Paraliobacillus 属的一个成员，与 Paraliobacillus quinghaiensis YIM-C158T 和 Paraliobacillus ryukyuensis O15-7T 的 16S rRNA 基因序列相似度分别为 96.2%和 95.3%。DNA G+C 含量为 39.6mol%。该菌株含有 MK-7 作为唯一的呼吸醌，anteiso-C15：0、C16：0、iso-C15：0 和 iso-C16：0 作为主要的细胞脂肪酸，其极性脂图谱由二磷脂酰甘油、磷脂酰乙醇胺、三种糖脂和四种未知磷脂组成。基于其系统发育位置、表型特征和化学分类学特征，建议菌株 126C4T 代表 Paraliobacillus 属的一个新种，命名为 Paraliobacillus sediminis sp. nov.。模式菌株为 126C4T（=KCTC 33762T=MCCC 1H00136T）。
