Tsujimoto H, Noda Y, Ishikawa K, Nakamura H, Fukasawa M, Sakakibara I, Sasagawa A, Honjo S, Hayami M
Cancer Res. 1987 Jan 1;47(1):269-74.
Proviral integration of a simian retrovirus highly homologous to human T-cell leukemia virus type I was examined in cellular DNAs extracted from primary peripheral blood lymphocytes of 31 adult African green monkeys (Cercopithecus aethiops) that were seropositive for simian T-cell leukemia virus type I (STLV-I). Among these monkeys, one case with overt leukemia, showing pleomorphic leukemia cells similar to those in human adult T-cell leukemia (ATL), and five cases in a preleukemic state of ATL-like disease were found. Judging from the integration site of the provirus genome, primary lymphocytes of these leukemic or preleukemic cases contained monoclonally proliferated STLV-I-infected cells, whereas lymphocytes of other seropositive monkeys without hematological abnormalities were polyclonal, and those of seronegative monkeys did not contain the provirus. The restriction patterns with PstI ans SstI of most STLV-I proviruses were identical to those of the previous isolate from this species, but in three monkeys there was a deletion of one PstI site. From the correlation of the development of simian ATL-like disease with the monoclonal integration of the STLV-I provirus genome, it should be indicated that STLV-I has similar leukemogenicity to human T-cell leukemia virus type I, and so STLV-I infection in African green monkeys will be useful as an animal model of human ATL.
在从31只成年非洲绿猴（猕猴）的外周血淋巴细胞中提取的细胞DNA中，检测了一种与人类I型T细胞白血病病毒高度同源的猿猴逆转录病毒的前病毒整合情况。这些非洲绿猴的I型猿猴T细胞白血病病毒（STLV-I）血清学检测呈阳性。在这些猴子中，发现了1例患有明显白血病的病例，其白血病细胞形态多样，与人类成人T细胞白血病（ATL）的细胞相似，还有5例处于ATL样疾病的白血病前期状态。从病毒前基因组的整合位点判断，这些白血病或白血病前期病例的原代淋巴细胞含有单克隆增殖的STLV-I感染细胞，而其他无血液学异常的血清学阳性猴子的淋巴细胞是多克隆的，血清学阴性猴子的淋巴细胞则不含该前病毒。大多数STLV-I前病毒的PstI和SstI限制性酶切图谱与该物种先前分离株的图谱相同，但在3只猴子中，有一个PstI位点缺失。从猿猴ATL样疾病的发生与STLV-I前病毒基因组单克隆整合的相关性来看，应该表明STLV-I与人类I型T细胞白血病病毒具有相似的致白血病性，因此非洲绿猴中的STLV-I感染将作为人类ATL的动物模型具有重要价值。
