Liao Han-Tsung, Chen Jyh-Ping, Lee Ming-Yih
Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan.
Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan.
Materials (Basel). 2013 Oct 25;6(11):4911-4929. doi: 10.3390/ma6114911.
Three-dimensional porous polycaprolactone (PCL) scaffolds with consistent inter-pore channels, 83% porosity and 300-400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP) to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs). The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differentiation capability of PASCs in osteogenic medium toward the osteoblast lineage, judging from elevated alkaline phosphatase activity and up-regulated osteogenic genes expression. For study, a 3 cm × 3 cm mandible defect was created in pigs and reconstructed by implanting acellular PCL scaffolds or PCL/PRP/PASCs constructs. Both groups showed new bone formation, however, the new bone volume was 5.1 times higher for PCL/PRP/PASCs 6 months post-operation. The bone density was less and loose in the acellular PCL group and the Young's modulus was only 29% of normal bone. In contrast, continued and compact bone formation was found in PCL/PRP/PASCs and the Young's modulus was 81% that of normal bone. Masson's trichrome stain, immunohistochemical analysis of osteocalcin and collagen type I also confirmed new bone formation.
通过选择性激光烧结制备了具有一致孔间通道、孔隙率为83%且孔径为300 - 400μm的三维多孔聚己内酯（PCL）支架。将PCL支架与富血小板血浆（PRP）结合形成生物活性复合材料，并使用猪脂肪来源干细胞（PASC）研究其在骨组织工程中的潜在应用。从碱性磷酸酶活性升高和成骨基因表达上调判断，PCL/PRP/PASC构建体显示出提高的细胞接种效率，并协同提高了PASC在成骨培养基中向成骨细胞谱系的分化能力。为了进行研究，在猪身上制造了一个3 cm×3 cm的下颌骨缺损，并通过植入脱细胞PCL支架或PCL/PRP/PASC构建体进行重建。两组均显示有新骨形成，然而，术后6个月PCL/PRP/PASC组的新骨体积高出5.1倍。脱细胞PCL组的骨密度较低且疏松，杨氏模量仅为正常骨的29%。相比之下，PCL/PRP/PASC组发现有持续且致密的骨形成，杨氏模量为正常骨的81%。Masson三色染色、骨钙素和I型胶原的免疫组织化学分析也证实了新骨形成。
