Rostykus Manon, Moser Christophe
Opt Express. 2017 Jul 10;25(14):16652-16659. doi: 10.1364/OE.25.016652.
Current compact lensless holographic microscopes are based on either multiple angle in-line holograms, multiple wavelength illumination or a combination thereof. Complex computational algorithms are necessary to retrieve the phase image which slows down the visualization of the image. Here we propose a simple compact lensless transmission holographic microscope with an off-axis configuration which simplifies considerably the computational processing to visualize the phase images and opens the possibility of real time phase imaging using off the shelf smart phone processors and less than $3 worth of optics and detectors, suitable for broad educational dissemination. This is achieved using a side illumination and analog hologram gratings to shape the reference and signal illumination beams from one light source. We demonstrate experimentally imaging of cells with a field of view (FOV) of ~12mm, and a resolution of ~3.9μm.
当前的紧凑型无透镜全息显微镜基于多角度同轴全息图、多波长照明或两者的组合。需要复杂的计算算法来检索相位图像，这会减慢图像的可视化速度。在此，我们提出一种具有离轴配置的简单紧凑型无透镜透射全息显微镜，它大大简化了用于可视化相位图像的计算处理，并开启了使用现成的智能手机处理器以及价值不到3美元的光学器件和探测器进行实时相位成像的可能性，适用于广泛的教育传播。这是通过侧面照明和模拟全息光栅来塑造来自一个光源的参考光束和信号照明光束来实现的。我们通过实验展示了对细胞的成像，其视场（FOV）约为12mm，分辨率约为3.9μm。
