紧凑型在线无透镜数字全息显微镜。
Compact in-line lensfree digital holographic microscope.
机构信息
Laboratory of Applied Photonics Devices, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Biomedical Imaging Group, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Univ Lyon, Univ Lyon1, Ens de Lyon, CNRS, Centre de Recherche Astrophysique de Lyon UMR5574, F-69230 Saint-Genis-Laval, France.
出版信息
Methods. 2018 Mar 1;136:17-23. doi: 10.1016/j.ymeth.2017.11.008. Epub 2017 Nov 21.
Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm compared to several hundred μm) and more compact setups that traditional DHM which have high quality microscope objectives. In this article, a lensfree DHM is presented using a side illumination technique in order to further reduce the device size. Its practical use is described and results on a transparent (phase only) sample are shown.
相衬成像提供强度对比，可用于观察透明样本，如生物学样本，而无需任何染色。其中，数字全息显微镜（DHM）是一种众所周知的定量相方法。DHM 的无透镜实现具有提供大视场（与几百微米相比，为几毫米）和更紧凑设置的附加优势，而传统的 DHM 则具有高质量的显微镜物镜。本文提出了一种使用侧向照明技术的无透镜 DHM，以进一步减小器件尺寸。描述了其实际应用，并展示了对透明（仅相位）样本的结果。
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