Regan C M
J Pharm Pharmacol. 1986 Nov;38(11):834-6. doi: 10.1111/j.2042-7158.1986.tb04504.x.
A rapid and simple drug conjugation procedure which utilizes the heterobifunctional reagent N-hydroxysuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate is described. With this protocol two drugs of diverse structure and solubility have been successfully haptenized. Rabbits immunized with these conjugates yielded drug-specific antibodies as judged by a solid phase radioimmunoassay. Furthermore, using this technique, no cross-reactivity could be detected. Such haptens also elicited an immune response in mice thus further extending their potential for the production of drug specific monoclonal antibodies.
本文描述了一种快速简单的药物偶联方法，该方法使用了异双功能试剂6-(4'-叠氮基-2'-硝基苯氨基)己酸N-羟基琥珀酰亚胺酯。通过此方案，两种结构和溶解性不同的药物已成功半抗原化。用这些偶联物免疫的兔子通过固相放射免疫测定法判断产生了药物特异性抗体。此外，使用该技术未检测到交叉反应性。这种半抗原在小鼠中也引发了免疫反应，从而进一步扩展了它们产生药物特异性单克隆抗体的潜力。
