Université de Tunis El-Manar, Faculté des Sciences de Tunis, Département de Chimie, Laboratoire de Chimie Analytique & Electrochimie (LR99ES15), campus universitaire de Tunis El Manar, 2092 Tunis El-Manar, Tunisia.
Polymer Institute, Slovak Academy of Sciences, Dúbravskácesta 9, 845 41 Bratislava, Slovakia.
Food Chem. 2016 Oct 15;209:274-8. doi: 10.1016/j.foodchem.2016.04.073. Epub 2016 Apr 19.
We report on the design of an amperometric tyrosinase-based biosensor using a self-assembled monolayer of ω-mercaptopropyl naphthoquinone on gold electrode as an electron mediator. Under optimal conditions (i.e. pH=7.4 and E=-0.35V vs. KCl), the chronoamperometric response of the naphthoquinone-modified bioelectrode to successive additions of phenol was evaluated. The biosensor exhibits sensitive bioelectrocatalytic response at a working potential of -0.35V vs. Ag/AgCl (sat.KCl), reaching the steady-state current within 40s after each addition of phenol solution with a range of 0-135μM and a limit of detection and quantification which are 0.019μM and 0.0633μM, respectively. The bioelectrode was used to determine the content in polyphenol in a local virgin olive oil.
我们报告了一种基于辣根过氧化物酶的安培生物传感器的设计，该传感器使用金电极上的ω-巯基丙基萘醌自组装单分子层作为电子媒介。在最佳条件下（即 pH=7.4 和 E=-0.35V 相对于 KCl），评估了萘醌修饰的生物电极对苯酚连续加入的计时安培响应。该生物传感器在相对于 Ag/AgCl（饱和 KCl）的工作电位-0.35V 下表现出灵敏的生物电化学催化响应，在每次加入苯酚溶液后 40s 内达到稳态电流，范围为 0-135μM，检测限和定量限分别为 0.019μM 和 0.0633μM。该生物电极用于测定当地初榨橄榄油中多酚的含量。
