Pandini J A, Pinto F G S, Scur M C, Santana C B, Costa W F, Temponi L G
Programa de Conservação e Manejo de Recursos Naturais, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
Departamento de Química, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Braz J Biol. 2018 Feb;78(1):53-60. doi: 10.1590/1519-6984.04116. Epub 2017 Jul 27.
The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. The GC-MS analyses allowed identifying 13 constituents, representing 96.52% of the essencial oil composition. The main compounds identified were α-zingiberene (34.48%), β-sesquiphellandrene (22.90%), and α-curcumene (16.17%). With respect to the antimicrobial activity, the essential oil was effective against all the microorganisms tested, except for the bacteria E. coli and K. pneumoniae, which were resistant to the action of the oil. From a general point of view, Gram-positive bacteria were more susceptible to the action of the essential oil than Gram-negative bacteria. The essential oil exhibited antioxidant potential.
精油是从植物化合物中提取的，具有抗菌和抗氧化特性。本研究的目的是：（a）使用气相色谱-质谱联用（GC-MS）方法测定昆氏古夷苏木精油的化学成分；（b）采用肉汤微量稀释法评估该精油对不同微生物的抗菌潜力，包括五种革兰氏阴性菌、四种革兰氏阳性菌和一种酵母菌；（c）使用DPPH（2,2-二苯基-1-苦基肼）自由基测定法测定该精油的抗氧化活性。GC-MS分析鉴定出13种成分，占精油成分的96.52%。鉴定出的主要化合物为α-姜黄烯（34.48%）、β-倍半水芹烯（22.90%）和α-姜黄烯（16.17%）。关于抗菌活性，该精油对所有测试微生物均有效，但大肠杆菌和肺炎克雷伯菌对其作用具有抗性。总体而言，革兰氏阳性菌比革兰氏阴性菌对精油的作用更敏感。该精油具有抗氧化潜力。
