Li Jian, Ma Jun, Jiang Tao, Wang Yanhuan, Wen Xuemei, Li Guozhu
School of Material Science and Chemical Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Tianjin Synthetic Material Research Institute, Tianjin 300220, China.
Materials (Basel). 2015 Sep 9;8(9):6004-6017. doi: 10.3390/ma8095286.
Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH₂-Alginate, is synthesized through an oxidation-amination-reduction process. The "catalysis function" ensures the as-prepared NH₂-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na₂SiO₃), and the "template function" cause microscopic phase separation in aqueous solution. The diameter of resultant NH₂-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH₂-Alginate. The size and morphology of obtained NH₂-Alginate/silica nanocomposite are correlated with the micelles. NH₂-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH₂-Alginate and cage effect of silica matrix.
受生物矿化启发，仿生聚合物-二氧化硅纳米复合材料在科研和技术应用方面都引起了广泛关注。在本研究中，通过氧化-胺化-还原过程合成了一种新型双功能聚合物NH₂-海藻酸盐。“催化功能”确保所制备的NH₂-海藻酸盐能从低浓度前驱体（Na₂SiO₃）诱导二氧化硅的仿生矿化，而“模板功能”则导致水溶液中的微观相分离。所得NH₂-海藻酸盐胶束在水溶液中的直径分布于100nm至1.5μm之间，并受NH₂-海藻酸盐合成过程的影响。所获得的NH₂-海藻酸盐/二氧化硅纳米复合材料的尺寸和形态与胶束相关。随后利用NH₂-海藻酸盐/二氧化硅纳米复合材料固定β-葡萄糖醛酸酶（GUS）。由于NH₂-海藻酸盐的缓冲作用和二氧化硅基质的笼效应，固定化GUS的耐苛刻条件性和长期储存稳定性得到显著提高。
