Wang Ying, Li Yang, Zhou Liang, Guo Lin
Tianjin University of Traditional Chinese Medicine, Tianjin, China.
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Acupunct Med. 2017;35(5):374-382. doi: 10.1136/acupmed-2016-011170. Epub 2017 Aug 9.
The mechanism of action underlying the putative effects of acupuncture in essential hypertension remains unknown but neuronal mechanisms have been explored most extensively to date. We aimed to explore the effects of manual acupuncture (MA) with manual stimulation on blood pressure (BP) and urine metabolites in the spontaneously hypertensive rat (SHR) model of hypertension.
After adaptive feeding (with a standard laboratory diet and distilled water) for 1 week, five normotensive Wistar Kyoto (WKY) rats with normal BP formed the control group. Ten SHRs were randomised into two groups (n=5 each), one of which received MA at ST9 for a total of 14 days (SHR+MA group). The other (SHR group) and the WKY control group underwent needle insertion without manual stimulation. BP was measured pre- and post-acupuncture in all groups and urinary metabolic profiles were investigated using metabonomics methods based on H nuclear magnetic resonance (NMR) spectroscopy coupled with principal component analysis, partial least squares discriminant analysis, and orthogonal to partial least squares discriminant analysis.
A total of 46 metabolites were clearly separated in the urine by H NMR. Loading plots showed significant changes in urinary metabolite contents after acupuncture treatment as well as significant differences between SHR and WKY rats. Acupuncture at ST9 in SHRs increased urine metabolites including α-ketoglutaric acid, N-acetyl glutamic acid, and betaine. Furthermore, systolic and diastolic BP, mean arterial pressure and heart rate were significantly reduced after acupuncture.
Acupuncture may decrease BP in SHRs by improving the metabolic disorder associated with hypertension.
针刺治疗原发性高血压的潜在作用机制尚不清楚，但迄今为止对神经机制的探索最为广泛。我们旨在探讨手动针刺（MA）结合手动刺激对自发性高血压大鼠（SHR）高血压模型血压（BP）和尿液代谢产物的影响。
适应性喂养（标准实验室饮食和蒸馏水）1周后，5只血压正常的Wistar Kyoto（WKY）大鼠组成对照组。10只SHR随机分为两组（每组n = 5），其中一组在ST9进行MA治疗，共14天（SHR + MA组）。另一组（SHR组）和WKY对照组进行针刺但不进行手动刺激。在所有组针刺前后测量血压，并使用基于氢核磁共振（NMR）光谱结合主成分分析、偏最小二乘判别分析和正交偏最小二乘判别分析的代谢组学方法研究尿液代谢谱。
通过氢核磁共振在尿液中清晰分离出总共46种代谢产物。载荷图显示针刺治疗后尿液代谢产物含量有显著变化，以及SHR和WKY大鼠之间存在显著差异。SHR在ST9针刺增加了尿液代谢产物，包括α - 酮戊二酸、N - 乙酰谷氨酸和甜菜碱。此外，针刺后收缩压和舒张压、平均动脉压和心率显著降低。
针刺可能通过改善与高血压相关的代谢紊乱来降低SHR的血压。
