在基因上被选择用于低抗体产生的小鼠中的肠道微生物群失调。

Gut dysbiosis in mice genetically selected for low antibody production.

作者信息

da Silva Santos Ana Carolina, Jensen José Ricardo, de Oliveira Silvio Luis, Rodrigues Josias

机构信息

Department of Microbiology and Immunology, Institute of Biosciences of the State University of São Paulo (UNESP), Campus de Rubião Junior, Botucatu, SP 18618-961 Brazil.

Laboratory of Immunogenetics, Butantan Institute, Av. Dr. Vital Brazil 1500, São Paulo, SP 05503-900 Brazil.

出版信息

Gut Pathog. 2017 Aug 7;9:43. doi: 10.1186/s13099-017-0193-x. eCollection 2017.

DOI:10.1186/s13099-017-0193-x

PMID:28794801

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5547450/

Abstract

BACKGROUND

Dysbiosis is linked to the cause of several human diseases, many of which having an immunity related component. This work investigated whether mice genetically selected for low or high antibody production display differences in intestinal bacterial communities, and consisted in the comparison of fecal 16SV6-V8 rDNA PCR amplicons resolved by temperature gradient gel electrophoresis (TGGE) of five each of low (LIII) and high (HIII) antibody producing mice. 16SV6 rDNA amplicons of 2 mice from each line were sequenced.

RESULTS

LIII mice were grouped in a single TGGE cluster, displayed a low α-diversity, and were distinguished by low Firmicutes/Bacteroidetes ratio.

CONCLUSION

The results suggest that genetically driven low antibody production in mice is associated with gut dysbiosis.

摘要

背景

微生物群落失调与多种人类疾病的病因相关，其中许多疾病具有免疫相关成分。本研究调查了基因选择为低抗体产生或高抗体产生的小鼠在肠道细菌群落上是否存在差异，包括对五只低抗体产生（LIII）小鼠和五只高抗体产生（HIII）小鼠的粪便16S V6 - V8 rDNA PCR扩增子进行温度梯度凝胶电泳（TGGE）分析，并对每个品系的两只小鼠的16S V6 rDNA扩增子进行测序。