Kao Wei-Chieh, Chen Yen-Wen, Chu Chia-Ho, Chang Wen-Hsin, Shiesh Shu-Chu, Wang Yu-Lin, Lee Gwo-Bin
Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
Biomicrofluidics. 2017 Jul 19;11(4):044105. doi: 10.1063/1.4995257. eCollection 2017 Jul.
Cardiovascular diseases (CVDs) cause more than 17 × 10 deaths worldwide on a yearly basis. Early diagnosis of CVDs is therefore of great need. The C-reactive protein (CRP) is an important biomarker for analyzing the risks of CVDs. In this work, CRP-specific aptamers with high sensitivity and specificity and field-effect-transistor (FET) devices were used to recognize and detect CRP by using an integrated microfluidic system automatically while consuming less volumes of reagents and samples (about 5 m). In order to package the FET device into the microfluidic chip, a new method to prevent liquid leakage was proposed. Sensitive detection of CRP has been demonstrated on the developed microfluidic system. It is the first time that aptamer-FET assays could be realized on an integrated microfluidic system. Experimental results showed that the aptamer-FET assay was capable of detecting CRP with concentrations ranging from 0.625 mg/l to 10.000 mg/l, which may be promising for early diagnosis of CVDs.
心血管疾病（CVDs）每年在全球导致超过17×10人死亡。因此，心血管疾病的早期诊断非常必要。C反应蛋白（CRP）是分析心血管疾病风险的重要生物标志物。在这项工作中，具有高灵敏度和特异性的CRP特异性适配体与场效应晶体管（FET）器件被用于通过集成微流控系统自动识别和检测CRP，同时消耗更少的试剂和样品体积（约5微升）。为了将FET器件封装到微流控芯片中，提出了一种防止液体泄漏的新方法。在开发的微流控系统上已证明对CRP的灵敏检测。这是首次在集成微流控系统上实现适配体-FET检测。实验结果表明，适配体-FET检测能够检测浓度范围为0.625毫克/升至10.000毫克/升的CRP，这对于心血管疾病的早期诊断可能很有前景。
