Sagi Orli, Berkowitz Anat, Codish Shlomi, Novack Victor, Rashti Aviv, Akad Fouad, Shemer-Avni Yonat
Clinical Parasitology.
Clinical Virology, and.
Open Forum Infect Dis. 2017 Feb 24;4(2):ofx037. doi: 10.1093/ofid/ofx037. eCollection 2017 Spring.
Rapid diagnosis of cutaneous leishmaniasis (CL) and identification of species is highly important for the disease management. In Israel, CL is caused mainly by and species.
We established an easy to handle point of care lesion-swabbing, combined with a highly sensitive multiplex real time PCR (multiplex qPCR) for accurate and rapid diagnosis of species.
Using three probes: one general for: Leishmania species, and two specific for , and , we screened 1783 clinical samples collected during two years. species was found in 1086 individuals, 1008 , and 70 . Eight samples positive for species only, were further tested using a second set of multiplex qPCR developed, and were found positive for and (2 and 6 samples, concomitantly).
Taken together, the test enabled diagnostics and better treatment of infections from the Old World (1078 samples) and the New World (8 samples), and the subtyping of the dominant strains in the region, as well as in returning travelers'.
皮肤利什曼病（CL）的快速诊断和病原体种类鉴定对疾病管理至关重要。在以色列，CL主要由[具体种类1]和[具体种类2]引起。
我们建立了一种易于操作的即时护理病灶拭子检测方法，并结合高灵敏度多重实时PCR（多重定量PCR），用于准确快速诊断[具体种类1]和[具体种类2]。
使用三种探针：一种针对利什曼原虫属的通用探针，以及两种分别针对[具体种类1]和[具体种类2]的特异性探针，我们对两年内收集的1783份临床样本进行了筛查。在1086名个体中发现了[具体种类1]，1008例感染[具体种类1]，70例感染[具体种类2]。仅对8份[具体种类2]呈阳性的样本，使用开发的第二套多重定量PCR进一步检测，发现其中2份同时对[具体种类1]呈阳性，6份同时对[具体种类2]呈阳性。
总体而言，该检测方法能够诊断并更好地治疗来自旧世界（1078份样本）和新世界（8份样本）的感染，以及该地区和归国旅行者中优势菌株的亚型分型。
