Oh Ami, Oh Byoung-Un
Department of Biology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Mol Biol Rep. 2017 Aug;44(4):359-363. doi: 10.1007/s11033-017-4117-0. Epub 2017 Aug 9.
Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for these two species were developed, and then characterized in 65 individuals (55 individuals of E. byunsanensis and 10 individuals of E. pungdoensis). The number of alleles per locus ranged from 2 to 9; the average number of alleles across all the loci scored 4.792. The unbiased diversity per locus ranged from 0.089 to 0.880; the unbiased diversity averaged over all the loci was 0.646. The developed markers were successfully amplified for three congeneric species, E. stellata, E. pinnatifida, and E. longistipitata. The markers developed in this study can provide a valuable and important tool for understanding genetic variations, population structures, evolutionary histories and phylogeography of E. byunsanensis, E. pungdoensis, and related species.
利用下一代测序技术，分离出了韩国特有物种粉萼玉凤花（Eranthis byunsanensis）和丰岛玉凤花（E. pungdoensis）（毛茛科）的叶绿体微卫星，以解决其分布模式和进化关系问题。为这两个物种开发了24个多态性叶绿体微卫星标记，并在65个个体（55个粉萼玉凤花个体和10个丰岛玉凤花个体）中进行了特征分析。每个位点的等位基因数从2到9不等；所有位点的平均等位基因数为4.792。每个位点的无偏多样性从0.089到0.880不等；所有位点的无偏多样性平均值为0.646。所开发的标记在三个同属物种，即星状玉凤花（E. stellata）、羽裂玉凤花（E. pinnatifida）和长梗玉凤花（E. longistipitata）中成功扩增。本研究中开发的标记可为了解粉萼玉凤花、丰岛玉凤花及相关物种的遗传变异、种群结构、进化历史和系统地理学提供有价值且重要的工具。
