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嗜铬粒细胞膜的质子转运三磷酸腺苷酶。活性位点位于最大的（70 kDa）亚基中。

Proton-translocating adenosine triphosphatase of chromaffin-granule membranes. The active site is in the largest (70 kDa) subunit.

作者信息

Percy J M, Apps D K

出版信息

Biochem J. 1986 Oct 1;239(1):77-81. doi: 10.1042/bj2390077.

DOI:10.1042/bj2390077
PMID:2879537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147241/
Abstract

The proton-translocating adenosine triphosphatase (ATPase) of bovine chromaffin granules contains up to five different polypeptides. Its activity is inhibited by N-ethylmaleimide, and ATP protects the enzyme from inhibition. After treatment of membranes with N-[2-3H]ethylmaleimide, only one polypeptide is strongly radiolabelled: this is the largest (70 kDa) subunit of the proton-translocating ATPase. This subunit therefore contains the ATP-hydrolysing site. Two-dimensional electrophoresis reveals heterogeneity in this polypeptide.

摘要

牛嗜铬颗粒的质子转运三磷酸腺苷酶（ATP酶）含有多达五种不同的多肽。其活性受到N-乙基马来酰亚胺的抑制，而ATP可保护该酶免受抑制。用N-[2-³H]乙基马来酰亚胺处理膜后，只有一种多肽被强烈放射性标记：这是质子转运ATP酶的最大（70 kDa）亚基。因此，该亚基包含ATP水解位点。二维电泳显示该多肽存在异质性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6a3/1147241/f5c8c4f306d9/biochemj00270-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6a3/1147241/824d74ba0979/biochemj00270-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6a3/1147241/f5c8c4f306d9/biochemj00270-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6a3/1147241/824d74ba0979/biochemj00270-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6a3/1147241/f5c8c4f306d9/biochemj00270-0084-a.jpg

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本文引用的文献

1
Partial purification of a tonoplast ATPase from corn coleoptiles.从玉米幼茎中部分纯化液泡膜 ATP 酶。
Plant Physiol. 1985 Jun;78(2):327-33. doi: 10.1104/pp.78.2.327.
2
The molecular organization of adrenal chromaffin granules.肾上腺嗜铬颗粒的分子结构
Neuroscience. 1980;5(11):1803-23. doi: 10.1016/0306-4522(80)90031-7.
3
The proton-translocating ATPase of the fungal plasma membrane.真菌质膜的质子转运ATP酶。
Experientia. 1996 Dec 15;52(12):1101-10. doi: 10.1007/BF01952108.
4
Effects of the sulfhydryl reagent N-ethylmaleimide on reserpine binding to the catecholamine transporter in chromaffin granule membranes.巯基试剂N-乙基马来酰亚胺对利血平与嗜铬粒细胞膜中儿茶酚胺转运体结合的影响。
Cell Mol Neurobiol. 1988 Jun;8(2):217-24. doi: 10.1007/BF00711247.
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A sodium/proton antiporter in chromaffin-granule membranes.嗜铬粒膜中的一种钠/质子反向转运体。
Biochem J. 1989 Jan 15;257(2):499-507. doi: 10.1042/bj2570499.
6
Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.液泡型H⁺转运ATP酶的拓扑结构：嗜铬粒膜ATP酶I
Biochem J. 1989 Oct 1;263(1):81-8. doi: 10.1042/bj2630081.
7
Structure, molecular genetics, and evolution of vacuolar H+-ATPases.液泡H⁺-ATP酶的结构、分子遗传学及进化
J Bioenerg Biomembr. 1989 Oct;21(5):553-71. doi: 10.1007/BF00808113.
8
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Biochem J. 1990 Aug 15;270(1):57-61. doi: 10.1042/bj2700057.
9
Structural conservation and functional diversity of V-ATPases.V-ATP酶的结构保守性与功能多样性
J Bioenerg Biomembr. 1992 Aug;24(4):407-14. doi: 10.1007/BF00762533.
Biochim Biophys Acta. 1981 Dec 30;639(3-4):197-223. doi: 10.1016/0304-4173(81)90010-0.
4
Isolation of a DCCD-binding protein from bovine chromaffin-granule membranes.
FEBS Lett. 1981 Jul 20;130(1):103-6. doi: 10.1016/0014-5793(81)80675-8.
5
Glycoproteins of the chromaffin granule membrane: separation by two-dimensional electrophoresis and identification by lectin binding.
J Neurochem. 1984 Nov;43(5):1243-52. doi: 10.1111/j.1471-4159.1984.tb05379.x.
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Inhibition of adenosine triphosphatase, 5-hydroxytryptamine transport and proton-translocation activities of resealed chromaffin-granule 'ghosts'.对重封的嗜铬粒蛋白“空壳”的三磷酸腺苷酶、5-羟色胺转运和质子转运活性的抑制作用
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Phase separation of integral membrane proteins in Triton X-114 solution.整合膜蛋白在Triton X-114溶液中的相分离。
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FEBS Lett. 1982 Nov 22;149(1):71-4. doi: 10.1016/0014-5793(82)81074-0.
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