Department of Organic Chemistry, Indian Institute of Science , Bangalore 560012, India.
Anal Chem. 2017 Oct 3;89(19):10376-10383. doi: 10.1021/acs.analchem.7b02344. Epub 2017 Sep 21.
A pyrene based amphiphilic receptor has been utilized in the nanomolar detection of uric acid at physiological pH in water. The compound shows a unique concentration-dependent modulation in optical response toward uric acid. In intramolecular/static excimer form (low concentration range), it displays a ratiometric response, while a "turn-off" sensing is observed specifically in the presence of intermolecular/dynamic excimer (high concentration range). The present protocol is then employed for the estimation of uric acid in blood serum samples of healthy individuals. Bland-Altman analysis in comparison to clinically approved uricase assay indicated the high accuracy of the present method. Additionally, the extent of insect infestation in stored grain samples was determined by measuring the uric acid content of their aqueous extracts. Low-cost color strips were developed for on-site detection of uric acid without involving any sophisticated instrument or trained personnel.
一种基于芘的两亲性受体已被用于在生理 pH 值的水中对尿酸进行纳摩尔级别的检测。该化合物对尿酸表现出独特的浓度依赖性光学响应调节。在分子内/静态激基缔合物形式（低浓度范围）下，它显示出比率响应，而在存在分子间/动态激基缔合物（高浓度范围）时则观察到“关闭”感应。然后，该方案用于估算健康个体血清样本中的尿酸。与临床批准的尿酸酶测定法进行的 Bland-Altman 分析表明，该方法具有很高的准确性。此外，通过测量其水溶液提取物中的尿酸含量，确定了储存谷物样品中的虫害程度。开发了低成本的色带，用于现场检测尿酸，而无需涉及任何复杂的仪器或训练有素的人员。
