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基于家禽胶原蛋白的杂化水凝胶的开发与特性研究及其在骨再生中的应用。

Development and characterization of poultry collagen-based hybrid hydrogels for bone regeneration.

机构信息

Fellow PhD degree. Universidade Federal do Ceará - Postgraduate Program in Biotechnology of Natural Resources - Fortaleza (CE), Brazil.

MSc. Universidade Federal do Ceará - Postgraduate Program in Biotechnology - Sobral (CE), Brazil.

出版信息

Acta Cir Bras. 2022 May 13;37(3):e370302. doi: 10.1590/acb370302. eCollection 2022.

DOI:10.1590/acb370302
PMID:35584534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9109989/
Abstract

PURPOSE

Poultry by-products can contribute as an innovative natural source for the development of composites based on polymers and minerals aiming at bone regeneration. The objective of this study was the physicochemical and biological characterization of collagen-based hydrogels crosslinked with ultraviolet (UV)-riboflavin.

METHODS

Pure hydrogels of 100% collagen (G1) or hybrid hydrogels, 90% collagen:10% apatite (G2), 90% collagen:10% nanokeratin (G3), and 90% collagen:5% apatite:5% nanokeratin (G4) were characterized by scanning electron microscope, Fourier-transform infrared spectroscopy, differential scanning calorimetry, swelling degree and quali-quantitative histological analysis. Ectopic implantation in subcutaneous tissue in mice at one, three and nine weeks allowed to assess the inflammation (neutrophils, lymphocytes, macrophages, and giant cells) and repair (neovascularization, and connective tissue) to determine biocompatibility and the integrity of biomaterials to score their biodegradability. Histomorphometry on critical size defects in rat calvaria at one and three months evaluated the percentage of bone, connective tissue, and biomaterials in all groups.

RESULTS

The hydrogels presented porous microstructure, water absorption and physicochemical characteristics compatible with their polymeric and/or mineral composition. All materials exhibited biocompatibility, biodegradability, and low osteoconductivity. G2 showed greater density of new bone and biomaterial than the G1, G3 and G4.

CONCLUSIONS

The collagen-apatite group formulation suggests potential for development as osteopromoting membrane.

摘要

目的

家禽副产品可以作为一种创新的天然来源，用于开发基于聚合物和矿物质的复合材料，以促进骨再生。本研究的目的是对用紫外线（UV）-核黄素交联的基于胶原蛋白的水凝胶进行物理化学和生物学特性分析。

方法

纯胶原蛋白水凝胶（G1）或杂化水凝胶，90%胶原蛋白：10%磷灰石（G2）、90%胶原蛋白：10%纳米角蛋白（G3）和 90%胶原蛋白：5%磷灰石：5%纳米角蛋白（G4）的物理化学和生物学特性通过扫描电子显微镜、傅里叶变换红外光谱、差示扫描量热法、溶胀度和定性-定量组织学分析进行了表征。在小鼠的皮下组织中进行异位植入，在 1、3 和 9 周时评估炎症（中性粒细胞、淋巴细胞、巨噬细胞和巨细胞）和修复（新生血管形成和结缔组织），以确定生物相容性和生物材料的完整性，以评估其生物降解性。在大鼠颅骨临界尺寸缺陷处进行 1 个月和 3 个月的组织形态计量学评估，以确定所有组中骨、结缔组织和生物材料的百分比。

结果

水凝胶具有多孔微观结构、吸水性和物理化学特性，与它们的聚合物和/或矿物质组成相兼容。所有材料均表现出生物相容性、生物降解性和低骨诱导性。G2 显示出比 G1、G3 和 G4 更高的新骨和生物材料密度。

结论

胶原蛋白-磷灰石组配方具有作为促骨形成膜的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/ab44f8669dee/1678-2674-acb-37-3-e370302-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/e4ae8923de35/1678-2674-acb-37-3-e370302-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/83755c794456/1678-2674-acb-37-3-e370302-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/f6b42d385bcd/1678-2674-acb-37-3-e370302-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/1d044c56261a/1678-2674-acb-37-3-e370302-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/4109dd87894b/1678-2674-acb-37-3-e370302-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/cdd18170db76/1678-2674-acb-37-3-e370302-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/ac45a1ed0312/1678-2674-acb-37-3-e370302-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/ab44f8669dee/1678-2674-acb-37-3-e370302-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/e4ae8923de35/1678-2674-acb-37-3-e370302-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/83755c794456/1678-2674-acb-37-3-e370302-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/f6b42d385bcd/1678-2674-acb-37-3-e370302-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/1d044c56261a/1678-2674-acb-37-3-e370302-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/4109dd87894b/1678-2674-acb-37-3-e370302-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/cdd18170db76/1678-2674-acb-37-3-e370302-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/ac45a1ed0312/1678-2674-acb-37-3-e370302-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3a6/9109989/ab44f8669dee/1678-2674-acb-37-3-e370302-gf08.jpg

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