1 EA 2496 Laboratory Orofacial Pathologies, Imagery and Biotherapies, Dental School and Life imaging Platform (PIV), University Paris Descartes Sorbonne Paris Cité, Montrouge, France.
2 AP-HP, Departments of Odontology, University Hospitals Albert Chenevier, Louis Mourier and Bretonneau "National Rare Diseases Center Metabolism Phosphorus and Calcium" and Charles Foix, Paris, France.
J Dent Res. 2017 Nov;96(12):1406-1413. doi: 10.1177/0022034517725523. Epub 2017 Aug 10.
Cell-based partial pulp regeneration is one of the promising approaches to obtain newly formed functional dentin-pulp complex. It relies on the preservation of the healthy tissue while regenerating the damaged pulp. The aim of this study was to investigate whether this regenerative process could be achieved by implanting porcine dental pulp cells (pDPCs) in pulp defects in the minipig. By split-mouth model, self-assembling injectable nanopeptide hydrogel, with and without pDPCs, was implanted after cameral pulpotomy in premolars and molars. At day 21 after surgery, 3-dimensional morphometric characterization, Masson's trichrome staining, and immunolabeling for DSP and BSP (dentin sialoprotein and bone sialoprotein) were performed on treated teeth. This study demonstrated no pulp regeneration but systematic reparative dentinogenesis. In fact, regardless of the presence of pDPCs in the scaffold, an osteodentin bridge-the microarchitecture of which significantly differed from the native dentin-was systematically obtained. Furthermore, the presence of pDPCs significantly affected the microstructure of the dentin bridges. In the radicular area of each treated tooth, hyperemia in the remaining pulp and external root resorptions were observed. Under the conditions tested in this work, pulp regeneration was not achieved, which highlights the need of further investigations to develop favorable regenerative microenvironment.
基于细胞的部分牙髓再生是获得新形成的功能性牙本质-牙髓复合体的有前途的方法之一。它依赖于在再生受损牙髓的同时保留健康组织。本研究的目的是研究通过在小型猪的牙髓缺损中植入猪牙髓细胞（pDPC）是否可以实现这种再生过程。通过劈裂口腔模型，在切牙和磨牙的冠髓切断术后，分别植入含有和不含有 pDPC 的自组装可注射纳米肽水凝胶。术后 21 天，对处理后的牙齿进行三维形态计量学特征、Masson 三色染色和 DSP（牙本质涎蛋白）和 BSP（骨涎蛋白）免疫标记。本研究表明没有牙髓再生，但有系统的修复性牙本质形成。事实上，无论支架中是否存在 pDPC，都会系统地获得骨-牙本质桥——其微观结构与天然牙本质显著不同。此外，pDPC 的存在显著影响了牙本质桥的微观结构。在每个处理牙的根尖区域，观察到剩余牙髓充血和外部牙根吸收。在本工作中测试的条件下，未实现牙髓再生，这突出表明需要进一步研究以开发有利的再生微环境。
