Jo Woo-Ri, Park Hye-Jin
Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong gu Seongnam-si Gyeonggi-do, 461-701, Republic of Korea.
Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong gu Seongnam-si Gyeonggi-do, 461-701, Republic of Korea.
J Nutr Biochem. 2017 Oct;48:103-111. doi: 10.1016/j.jnutbio.2017.06.010. Epub 2017 Jun 30.
Fisetin (3,7,3',4'-tetrahydroxyflavone), a naturally occurring bioactive flavonoid, has been shown to inhibit inflammation. However, little is known about the effect of fisetin on immunoglobulin E (IgE)-mediated allergic responses. In this study, the effect of fisetin on rat basophilic leukemia (RBL-2H3) cell-mediated allergic reactions was investigated. Fisetin inhibited β-hexosaminidase release and decreased the level of interleukin-4 and tumor necrosis factor-α mRNA in IgE/antigen (IgE/Ag)-stimulated RBL-2H3 cells. To elucidate the antiallergic mechanism, we examined the levels of signaling molecules responsible for degranulation and release of inflammatory cytokines. Fisetin decreased the levels of activated spleen tyrosine kinase, Gab2 proteins, linker of activated T cells, extracellular signal-related kinase 1/2 in the IgE/Ag-stimulated RBL2H3 cells, and NFκB and STAT3 proteins activated in the ear tissue of mice with passive cutaneous anaphylaxis (PCA). In addition, fisetin significantly lowered of FcɛRI α-subunit mRNA expression. Consistent with the cellular data, fisetin markedly suppressed RBL-2H3 cell-dependent PCA in IgE/Ag-sensitized mice. These results suggest that fisetin may have potential as a therapeutic agent for the treatment of allergic diseases.
漆黄素（3,7,3',4'-四羟基黄酮）是一种天然存在的生物活性黄酮类化合物，已被证明具有抗炎作用。然而，关于漆黄素对免疫球蛋白E（IgE）介导的过敏反应的影响知之甚少。在本研究中，研究了漆黄素对大鼠嗜碱性白血病（RBL-2H3）细胞介导的过敏反应的影响。漆黄素抑制β-己糖胺酶释放，并降低IgE/抗原（IgE/Ag）刺激的RBL-2H3细胞中白细胞介素-4和肿瘤坏死因子-α mRNA的水平。为了阐明抗过敏机制，我们检测了负责脱颗粒和炎症细胞因子释放的信号分子水平。漆黄素降低了IgE/Ag刺激的RBL2H3细胞中活化的脾酪氨酸激酶、Gab2蛋白、活化T细胞连接蛋白、细胞外信号相关激酶1/2的水平，以及被动皮肤过敏反应（PCA）小鼠耳部组织中活化的NFκB和STAT3蛋白的水平。此外，漆黄素显著降低了FcɛRI α亚基mRNA的表达。与细胞数据一致，漆黄素显著抑制了IgE/Ag致敏小鼠中RBL-2H3细胞依赖性PCA。这些结果表明，漆黄素可能具有作为治疗过敏性疾病的治疗剂的潜力。
