School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju 500-712, Republic of Korea.
Department of Earth and Environmental Engineering, Columbia University, 500 West 120th Street, New York, NY 10027, United States.
Bioresour Technol. 2018 Feb;249:635-643. doi: 10.1016/j.biortech.2017.10.073. Epub 2017 Oct 20.
This study examines the fate of twenty-three representative antibiotic resistance genes (ARGs) encoding tetracyclines, sulfonamides, quinolones, β-lactam antibiotics, macrolides, florfenicol and multidrug resistance during thermophilic aerobic digestion (TAD) of sewage sludge. The bacterial community, class 1 integrons (intI1) and four metal resistance genes (MRGs) were also quantified to determine the key drivers of changes in ARGs during TAD. At the end of digestion, significant decreases in the quantities of ARGs, MRGs and intI1 as well as 16S rRNA genes were observed. Partial redundancy analysis (RDA) showed that shifts in temperature were the key factors affecting a decrease in ARGs. Shifts in temperature led to decreased amounts of ARGs by reducing resistome and bacterial diversity, rather than by lowering horizontal transfer potential via intI1 or co-resistance via MRGs.
本研究考察了 23 种代表性抗生素耐药基因（ARGs）在污水污泥高温好氧消化（TAD）过程中的命运，这些基因编码四环素、磺胺类、喹诺酮类、β-内酰胺类抗生素、大环内酯类、氟苯尼考和多药耐药性。还定量了细菌群落、I 类整合子（intI1）和 4 种金属耐药基因（MRGs），以确定 TAD 过程中 ARGs 变化的关键驱动因素。在消化结束时，观察到 ARGs、MRGs 和 intI1 以及 16S rRNA 基因的数量显著减少。偏冗余分析（RDA）表明，温度的变化是影响 ARGs 减少的关键因素。温度的变化通过降低抗药性和细菌多样性，而不是通过降低 intI1 的水平转移潜力或通过 MRGs 的共抗性，导致 ARGs 数量减少。
