Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Tasmania, Australia.
Future Industries Institute, University of South Australia, Building X, Mawson Lakes Campus, GPO Box 2471, Adelaide, SA 5001, Australia.
Sci Rep. 2017 Aug 10;7(1):7847. doi: 10.1038/s41598-017-08423-x.
Polymerized High Internal Phase Emulsions (PolyHIPEs) were prepared using emulsion-templating, stabilized by an amphiphilic diblock copolymer prepared by reversible addition fragmentation chain transfer (RAFT) polymerization. The diblock copolymer consisted of a hydrophilic poly(ethylene glycol) methyl ether acrylate (PEO MA, average Mn 480) segment and a hydrophobic styrene segment, with a trithiocarbonate end-group. These diblock copolymers were the sole emulsifiers used in stabilizing "inverse" (oil-in-water) high internal phase emulsion templates, which upon polymerization resulted in a polyHIPE exhibiting a highly interconnected monolithic structure. The polyHIPEs were characterized by FTIR spectroscopy, BET surface area measurements, SEM, SEM-EDX, and TGA. These materials were subsequently investigated as stationary phase for high-performance liquid chromatography (HPLC) via in situ polymerization in a capillary format as a 'column housing'. Initial separation assessments in reversed-phase (RP) and hydrophilic interaction liquid chromatographic (HILIC) modes have shown that these polyHIPEs are decorated with different microenvironments amongst the voids or domains of the monolithic structure. Chromatographic results suggested the existence of RP/HILIC mixed mode with promising performance for the separation of small molecules.
聚合高内相比乳液（PolyHIPEs）是通过乳化模板法制备的，由通过可逆加成-断裂链转移（RAFT）聚合制备的两亲性嵌段共聚物稳定。该两亲嵌段共聚物由亲水性聚（乙二醇）甲基醚丙烯酸酯（PEO MA，平均 Mn 480）段和疏水性苯乙烯段组成，带有三硫代碳酸酯端基。这些两亲嵌段共聚物是唯一用于稳定“反相”（油包水）高内相比乳液模板的乳化剂，聚合后得到的聚 HIPEs 呈现出高度互连的整体结构。通过傅里叶变换红外光谱（FTIR）、BET 比表面积测量、SEM、SEM-EDX 和 TGA 对聚 HIPEs 进行了表征。随后，通过在毛细管形式中的原位聚合作为“柱壳”，将这些材料作为高效液相色谱（HPLC）的固定相进行了研究。反相（RP）和亲水相互作用液相色谱（HILIC）模式下的初始分离评估表明，这些聚 HIPEs 在整体结构的空隙或域中具有不同的微环境。色谱结果表明存在 RP/HILIC 混合模式，对小分子的分离具有有前景的性能。
