Chen Hui-Hua, Zhao Pei, Tian Jing, Guo Wei, Xu Ming, Zhang Chen, Lu Rong
Department of Pathology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Public Experiment Platform, School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Evid Based Complement Alternat Med. 2017;2017:2915247. doi: 10.1155/2017/2915247. Epub 2017 Jul 17.
Guizhi Gancao Decoction (GGD), a traditional Chinese medical recipe, has been widely used in the treatment of cardiovascular diseases in China for centuries. The present study was carried out to determine whether GGD exerts direct protective effects against pressure overload-induced heart failure. Moreover, we investigated whether GGD affects tubulin expression and posttranslational modifications. We demonstrated that GGD ameliorated TAC caused cardiac hypertrophy by gravimetric and echocardiography analysis in C57BL/6 mice. We found that GGD abrogated TAC-induced myocardium fibrosis by Masson's staining and collagen volume fraction (CVF) analysis. By using pressure-volume hemodynamic measurements, we found that GGD prevented TAC-induced cardiac systolic and diastolic dysfunction. Immunoblotting and immunofluorescent analysis revealed that GGD abrogated TAC-induced detyrosination and acetylation abnormalities on microtubules. Our present study demonstrated potential therapeutic effects of GGD against pressure overload-induced heart failure.
桂枝甘草汤（GGD）是一种传统中药方剂，几个世纪以来在中国一直被广泛用于治疗心血管疾病。本研究旨在确定GGD是否对压力超负荷诱导的心力衰竭具有直接保护作用。此外，我们研究了GGD是否影响微管蛋白的表达和翻译后修饰。我们通过重量分析和超声心动图分析证明，GGD改善了C57BL/6小鼠中主动脉缩窄（TAC）引起的心脏肥大。我们通过Masson染色和胶原容积分数（CVF）分析发现，GGD消除了TAC诱导的心肌纤维化。通过压力-容积血流动力学测量，我们发现GGD预防了TAC诱导的心脏收缩和舒张功能障碍。免疫印迹和免疫荧光分析显示，GGD消除了TAC诱导的微管上脱酪氨酸化和乙酰化异常。我们目前的研究证明了GGD对压力超负荷诱导的心力衰竭具有潜在治疗作用。
