从骨髓中去除红细胞：Spectra Optia BMC 进行 50 次临床规模去除后的性能评估。
Erythrocyte depletion from bone marrow: performance evaluation after 50 clinical-scale depletions with Spectra Optia BMC.
机构信息
Institute Frankfurt, German Red Cross Blood Service Baden-Württemberg-Hessen, Sandhofstr. 1, 60528, Frankfurt, Germany.
Division of Stem Cell Transplantation, Department of Medicine II, Goethe University, Frankfurt, Germany.
出版信息
J Transl Med. 2017 Aug 11;15(1):174. doi: 10.1186/s12967-017-1277-6.
BACKGROUND
Red blood cell (RBC) depletion is a standard graft manipulation technique for ABO-incompatible bone marrow (BM) transplants. The BM processing module for Spectra Optia, "BMC", was previously introduced. We here report the largest series to date of routine quality data after performing 50 clinical-scale RBC-depletions.
METHODS
Fifty successive RBC-depletions from autologous (n = 5) and allogeneic (n = 45) BM transplants were performed with the Spectra Optia BMC apheresis suite. Product quality was assessed before and after processing for volume, RBC and leukocyte content; RBC-depletion and stem cell (CD34+ cells) recovery was calculated there from. Clinical engraftment data were collected from 26/45 allogeneic recipients.
RESULTS
Median RBC removal was 98.2% (range 90.8-99.1%), median CD34+ cell recovery was 93.6%, minimum recovery being 72%, total product volume was reduced to 7.5% (range 4.7-23.0%). Products engrafted with expected probability and kinetics. Performance indicators were stable over time.
DISCUSSION
Spectra Optia BMC is a robust and efficient technology for RBC-depletion and volume reduction of BM, providing near-complete RBC removal and excellent CD34+ cell recovery.
背景
红细胞（RBC）去除是用于 ABO 不相容骨髓（BM）移植的标准移植物处理技术。Spectra Optia 的 BM 处理模块“BMC”之前已经介绍过。我们在此报告了迄今为止进行 50 例临床规模 RBC 去除后常规质量数据的最大系列。
方法
使用 Spectra Optia BMC 单采仪对 5 例自体（n=5）和 45 例同种异体（n=45）BM 移植进行了 50 次连续 RBC 去除。在处理前后评估产品质量，包括体积、RBC 和白细胞含量；由此计算 RBC 去除率和干细胞（CD34+细胞）回收率。从 26/45 例同种异体受者中收集了临床植入数据。
结果
中位 RBC 去除率为 98.2%（范围 90.8-99.1%），中位 CD34+细胞回收率为 93.6%，最低回收率为 72%，总产品体积减少至 7.5%（范围 4.7-23.0%）。产品按预期概率和动力学植入。性能指标随时间保持稳定。
讨论
Spectra Optia BMC 是一种用于 RBC 去除和 BM 体积减少的强大且高效的技术，可提供近乎完全的 RBC 去除和出色的 CD34+细胞回收率。
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