Transfusion Medicine and Haemostaseology Department, University Hospital Erlangen, Erlangen, Germany.
Vox Sang. 2014 Apr;106(3):248-55. doi: 10.1111/vox.12102. Epub 2013 Oct 24.
Leukapheresis is an important source for mononuclear cells (MNCs) used in adoptive immunotherapies. Differences in the apheresis technology concerning physical conditions during cell separation and the optical detection system can affect the product's cellular content.
In a paired analysis, twenty healthy non-cytokine-stimulated donors underwent MNC collection at the Spectra Optia (Terumo BCT, Lakewood, CO, USA) and the COM.TEC (Fresenius Kabi, Bad Homburg, Germany) device. In twelve donors, apheresis was additionally performed with the Amicus (Fenwal Inc., Lake Zurich, IL, USA). Donor response to leukapheresis and product composition was compared.
Mean yields of CD14+ (CD3+) cells were 1·64±0·70x10(9) (2·36±0·96×10(9)) in the Spectra Optia, 1·45±0·50×10(9) (3·03±1·04×10(9)) in the COM.TEC and 1·20±0·37×10(9) (2·80±1·00×10(9)) in the Amicus products, respectively. The Spectra Optia collected significantly more CD14+ monocytes than the Amicus and significantly less CD3+ T cells than the COM.TEC (P=0·002 and P=0·021). Apheresis products of the Spectra Optia showed the significantly lowest red blood cell yields while the Amicus generated products with the significantly lowest platelet contents.
Leukaphereses with the three devices resulted in almost equal total MNC yields. MNC products of the Spectra Optia and the Amicus could be used in preference for the monocyte enrichment by the Elutra system and the leukapheresis procedures could be also favourably applied in patients with low platelet counts. The COM.TEC is more efficient in monocyte and T-cell collection with the disadvantage of high residual non-target cell content in the products.
白细胞分离术是过继免疫治疗中使用的单核细胞（MNC）的重要来源。细胞分离过程中的物理条件和光学检测系统的差异会影响产品的细胞含量。
在一项配对分析中，二十名健康的非细胞因子刺激供者在 Spectra Optia（Terumo BCT，Lakewood，CO，USA）和 COM.TEC（Fresenius Kabi，Bad Homburg，Germany）仪器上进行 MNC 采集。在十二名供者中，还用 Amicus（Fenwal Inc.，Lake Zurich，IL，USA）进行了白细胞分离术。比较了供者对白细胞分离术的反应和产品组成。
Spectra Optia 的 CD14+（CD3+）细胞平均产量为 1.64±0.70x10(9)（2.36±0.96×10(9)），COM.TEC 为 1.45±0.50x10(9)（3.03±1.04×10(9)），Amicus 为 1.20±0.37x10(9)（2.80±1.00×10(9)）。Spectra Optia 收集的 CD14+单核细胞明显多于 Amicus，而 CD3+T 细胞明显少于 COM.TEC（P=0.002 和 P=0.021）。Spectra Optia 的白细胞分离术产物红细胞产量最低，而 Amicus 生成的血小板含量最低。
三种仪器的白细胞分离术几乎产生了相同的总 MNC 产量。Spectra Optia 和 Amicus 的 MNC 产品可以通过 Elutra 系统优先用于单核细胞富集，并且白细胞分离术也可以在血小板计数低的患者中有利地应用。COM.TEC 在收集单核细胞和 T 细胞方面更有效，但产品中残留的非靶细胞含量较高。
