Kumar Sachin, Geiger Hartmut
Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Research Foundation, Cincinnati, OH 45229, USA.
Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Research Foundation, Cincinnati, OH 45229, USA; Institute of Molecular Medicine, Ulm University, Ulm, Germany; Aging Research Center, Ulm University, Ulm, Germany.
Trends Mol Med. 2017 Sep;23(9):799-819. doi: 10.1016/j.molmed.2017.07.003. Epub 2017 Aug 8.
Hematopoietic stem cell (HSC) transplantation can restore a new functional hematopoietic system in recipients in cases where the system of the recipient is not functional or for example is leukemic. However, the number of available donor HSCs is often too low for successful transplantation. Expansion of HSCs and thus HSC self-renewal ex vivo would greatly improve transplantation therapy in the clinic. In vivo, HSCs expand significantly in the niche, but establishing protocols that result in HSC expansion ex vivo remains challenging. In this review we discuss current knowledge of niche biology, the intrinsic regulators of HSC self-renewal in vivo, and introduce novel niche-informed strategies of HSC expansion ex vivo.
造血干细胞（HSC）移植能够在受体的造血系统失去功能或发生白血病等情况下，为受体重建一个全新的功能性造血系统。然而，可用的供体造血干细胞数量往往过低，难以成功进行移植。在体外扩增造血干细胞并实现其自我更新，将极大地改善临床移植治疗。在体内，造血干细胞在特定微环境中会显著扩增，但建立能够在体外实现造血干细胞扩增的方案仍然具有挑战性。在这篇综述中，我们讨论了目前关于特定微环境生物学、体内造血干细胞自我更新的内在调节因子的知识，并介绍了基于特定微环境的新型体外造血干细胞扩增策略。
