Carlson J R, Mertens S C, Yee J L, Gardner M B, Watson-Williams E J, Ghrayeb J, Jennings M B, Biggar R J
Lancet. 1987 Feb 14;1(8529):361-2. doi: 10.1016/s0140-6736(87)91730-2.
A new dot enzyme immunoassay (EIA) with a conserved portion of the envelope protein of the human immunodeficiency virus (HIV) as antigen has been designed for use in areas with few laboratory facilities and by personnel with little laboratory experience. Sera were tested in 263 subjects who had AIDS or AIDS-related complex or were at-risk or not-at-risk of AIDS from the USA, Africa, and Asia/Oceania. The dot EIA was 100% sensitive in the American subjects, and there were only 2 false negatives in the others, both of which were negative by commercial EIA. The test is simple to perform, economical, rapid (30 min), and stable.
一种新的斑点酶免疫测定法（EIA）已被设计出来，该方法以人类免疫缺陷病毒（HIV）包膜蛋白的保守部分作为抗原，供实验室设施较少地区且实验室经验不足的人员使用。对来自美国、非洲以及亚洲/大洋洲的263名患有艾滋病或艾滋病相关综合征、有感染艾滋病风险或无感染艾滋病风险的受试者的血清进行了检测。斑点EIA在美国受试者中灵敏度为100%，在其他受试者中仅有2例假阴性，这两例假阴性样本用商业EIA检测也呈阴性。该检测方法操作简单、经济、快速（30分钟）且稳定。
