Department of Mathematics and Computer Science, University of Antwerp, Antwerp, Belgium.
Biomedical Informatics Research Center Antwerp (Biomina), University of Antwerp/Antwerp University Hospital, Edegem, Belgium.
Mass Spectrom Rev. 2018 Sep;37(5):697-711. doi: 10.1002/mas.21544. Epub 2017 Sep 7.
Mass spectrometry is a highly complex analytical technique and mass spectrometry-based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.
质谱是一种非常复杂的分析技术，基于质谱的蛋白质组学实验可能会受到很大的变异性的影响，这成为获得准确和可重复结果的障碍。因此，全面系统的质量控制方法是对生成结果产生信心的必要要求。典型的质谱实验由多个不同的阶段组成，包括样品制备、液相色谱、质谱和生物信息学阶段。我们回顾了可能影响质谱实验结果的所有这些步骤中的变异性来源，并讨论了如何监测和纠正对实验结果的负面影响。此外，我们描述了如何将不同样本复杂度的专门质量控制样本纳入实验工作流程，以及如何使用它们来严格评估仪器性能的详细方面。
