Ren Luyao, Shi Leming, Zheng Yuanting
State Key Laboratory of Genetic Engineering, School of Life Sciences and Human Phenome Institute, Fudan University, Shanghai, 200438 China.
Shanghai Cancer Center, Fudan University, Shanghai, 200032 China.
Phenomics. 2024 Mar 6;4(5):487-521. doi: 10.1007/s43657-023-00153-7. eCollection 2024 Oct.
High-throughput technologies for multiomics or molecular phenomics profiling have been extensively adopted in biomedical research and clinical applications, offering a more comprehensive understanding of biological processes and diseases. Omics reference materials play a pivotal role in ensuring the accuracy, reliability, and comparability of laboratory measurements and analyses. However, the current application of omics reference materials has revealed several issues, including inappropriate selection and underutilization, leading to inconsistencies across laboratories. This review aims to address these concerns by emphasizing the importance of well-characterized reference materials at each level of omics, encompassing (epi-)genomics, transcriptomics, proteomics, and metabolomics. By summarizing their characteristics, advantages, and limitations along with appropriate performance metrics pertinent to study purposes, we provide an overview of how omics reference materials can enhance data quality and data integration, thus fostering robust scientific investigations with omics technologies.
用于多组学或分子表型组学分析的高通量技术已在生物医学研究和临床应用中广泛采用，能更全面地了解生物过程和疾病。组学参考物质在确保实验室测量和分析的准确性、可靠性及可比性方面发挥着关键作用。然而，目前组学参考物质的应用已暴露出几个问题，包括选择不当和利用不足，导致各实验室结果不一致。本综述旨在通过强调在组学各层面（包括（表观）基因组学、转录组学、蛋白质组学和代谢组学）具有充分特征的参考物质的重要性来解决这些问题。通过总结它们的特征、优势和局限性以及与研究目的相关的适当性能指标，我们概述了组学参考物质如何提高数据质量和数据整合，从而促进使用组学技术进行可靠的科学研究。
