基于金纳米粒子修饰的氧化石墨烯的 DNA 生物传感器用于检测乳腺癌生物标志物以进行早期诊断。
DNA biosensors based on gold nanoparticles-modified graphene oxide for the detection of breast cancer biomarkers for early diagnosis.
机构信息
Electroanal. Lab., Applied Organic Chemistry Department, National Research Centre, El Bohouth St., Dokki, 12622 Giza, Egypt.
Nanobiotechnology & Bioanalysis Group, Department of Chemical Engineering, Universitat Rovirai Virgili, 43007 Tarragona, Spain.
出版信息
Bioelectrochemistry. 2017 Dec;118:91-99. doi: 10.1016/j.bioelechem.2017.07.002. Epub 2017 Jul 14.
Two different DNA (ERBB2c and CD24c) modified gold nanoparticles and graphene oxide loaded on glassy carbon electrodes were prepared for early detection of breast cancer markers by electrochemical detection of HER2. Comparative study of ERBB2c and CD24c for the detection was carried out. A "sandwich-type" detection strategy was employed in this electrochemical DNA biosensor and its response was measured by amperometric detection. The electrochemical signal enhancement achieved via gold nanoparticles and grapheme oxide system allowed for sensitive detection of the breast cancer biomarker ERBB2 and the control marker CD24. The modified graphene oxide was characterised using Raman spectroscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy transmission electron microscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy. The various steps involved in the modification of a glassy carbon electrode with graphene oxide, gold nanoparticles and DNA probes, target and reporter probe were electrochemically characterised using cyclic voltammetry and electrochemical impedance spectroscopy. Using amperometric detection of a horse radish peroxidase label, detection limits of 0.16nM and 0.23nM were obtained with sensitivity 378nA/nM and 219nA/nM for ERBB2 andCD24 respectively.
两种不同的 DNA（ERBB2c 和 CD24c）修饰的金纳米粒子和负载在玻碳电极上的氧化石墨烯被制备用于通过电化学检测 HER2 来早期检测乳腺癌标志物。对 ERBB2c 和 CD24c 进行了检测比较研究。在这种电化学 DNA 生物传感器中采用了“三明治”检测策略，并通过安培检测测量其响应。金纳米粒子和氧化石墨烯系统的电化学信号增强允许对乳腺癌生物标志物 ERBB2 和对照标志物 CD24 进行敏感检测。使用拉曼光谱、紫外可见光谱、傅里叶变换红外光谱透射电子显微镜、扫描电子显微镜和能量色散 X 射线光谱对改性氧化石墨烯进行了表征。使用循环伏安法和电化学阻抗谱对玻碳电极上的氧化石墨烯、金纳米粒子和 DNA 探针、靶标和报告探针的修饰的各个步骤进行了电化学表征。使用辣根过氧化物酶标记的安培检测，对于 ERBB2 和 CD24，获得了 0.16nM 和 0.23nM 的检测限，灵敏度分别为 378nA/nM 和 219nA/nM。
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