Department of Microbiology, College of Life Sciences, Nanjing Agricultural University; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China.
Department of Microbiology, College of Life Sciences, Nanjing Agricultural University; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China
Appl Environ Microbiol. 2018 Jun 18;84(13). doi: 10.1128/AEM.00438-18. Print 2018 Jul 1.
is among the best known medicinal basidiomycetes due to its production of many pharmacologically active compounds. To study the regulatory networks involved in its growth and development, we analyzed the relationship between reactive oxygen species (ROS) and Ca signaling in the regulation of hyphal branching and ganoderic acid (GA) biosynthesis after Cu treatment. Our results revealed that Cu treatment decreased the distance between hyphal branches and increased the GA content and the intracellular levels of ROS and Ca Further research revealed that the Cu-induced changes in hyphal branch distance, GA content, and cytosolic Ca level were dependent on increases in cytosolic ROS. Our results also showed that increased cytosolic Ca could reduce cytosolic ROS by activating antioxidases and modulating Cu accumulation, resulting in feedback to adjust hyphal growth and GA biosynthesis. These results indicated that cytosolic ROS and Ca levels exert important cross talk in the regulation of hyphal growth and GA biosynthesis induced by Cu Taken together, our results provide a reference for analyzing the interactions among different signal transduction pathways with regard to the regulation of growth and development in other filamentous fungi., which is known as an important medicinal basidiomycete, is gradually becoming a model organism for studying environmental regulation and metabolism. In this study, we analyzed the relationship between reactive oxygen species (ROS) and Ca signaling in the regulation of hyphal branching and ganoderic acid (GA) biosynthesis under Cu stress. The results revealed that the Cu-induced changes in the hyphal branch distance, GA content, and cytosolic Ca level were dependent on increases in cytosolic ROS. Furthermore, the results indicated that increased cytosolic Ca could reduce cytosolic ROS levels by activating antioxidases and modulating Cu accumulation. The results in this paper indicate that there was important cross talk between cytosolic ROS and Ca levels in the regulation of hyphal growth and GA biosynthesis induced by Cu.
灵芝因其能产生多种具有药理活性的化合物而成为最著名的药用担子菌之一。为了研究其生长发育过程中涉及的调控网络，我们分析了活性氧（ROS）和钙信号在铜处理后调控菌丝分支和灵芝酸（GA）生物合成中的关系。我们的结果表明，铜处理降低了菌丝分支之间的距离，增加了 GA 含量和细胞内 ROS 和 Ca 的水平。进一步的研究表明，铜诱导的菌丝分支距离、GA 含量和胞质 Ca 水平的变化依赖于胞质 ROS 的增加。我们的结果还表明，增加胞质 Ca 可以通过激活抗氧化酶和调节 Cu 积累来降低胞质 ROS，从而产生反馈调节菌丝生长和 GA 生物合成。这些结果表明，胞质 ROS 和 Ca 水平在铜诱导的菌丝生长和 GA 生物合成调控中发挥重要的交叉对话作用。总之，我们的研究结果为分析其他丝状真菌生长发育调控中不同信号转导途径之间的相互作用提供了参考。
