酿酒酵母HIS4基因的正向调控相互作用。
Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.
作者信息
Lucchini G, Hinnebusch A G, Chen C, Fink G R
出版信息
Mol Cell Biol. 1984 Jul;4(7):1326-33. doi: 10.1128/mcb.4.7.1326-1333.1984.
Abstract
The role of cis- and trans-acting elements in the expression of HIS4 has been examined by using HIS4-lacZ fusions in which lacZ expression is dependent upon the HIS4 5' noncoding region. The cis-acting sequences involved in regulation were defined by studying the effects of the wild-type and various deletions and their revertants on regulation via the general control of amino acid biosynthesis. The role of trans-acting genes was analyzed by studying the regulation of the HIS4-lacZ fusions in strains carrying mutations in the GCN (AAS) or GCD (TRA) genes and in strains carrying the GCN genes on high-copy-number plasmids. These studies have led to the following conclusions. (i) HIS4 is positively regulated by the general control. (ii) At least one copy of the 5'TGACTC3' repeat at -136 is required in cis for this regulation. (iii) Both the GCN4 gene and at least one copy of the repeated sequence are required for expression at the repressed level. (iv) The open reading frames in the 5' noncoding region are not required in either cis or trans for the regulation of HIS4.
摘要
通过使用HIS4-lacZ融合体来研究顺式和反式作用元件在HIS4表达中的作用，其中lacZ的表达依赖于HIS4的5'非编码区。通过研究野生型、各种缺失及其回复突变体对氨基酸生物合成的一般控制的调节作用，确定了参与调节的顺式作用序列。通过研究携带GCN（AAS）或GCD（TRA）基因突变的菌株以及携带高拷贝数质粒上GCN基因的菌株中HIS4-lacZ融合体的调节情况，分析了反式作用基因的作用。这些研究得出了以下结论。（i）HIS4受到一般控制的正向调节。（ii）对于这种调节，顺式作用需要在-136处至少有一个5'TGACTC3'重复序列拷贝。（iii）GCN4基因和至少一个重复序列拷贝是在抑制水平表达所必需的。（iv）5'非编码区的开放阅读框在HIS4的调节中无论是顺式还是反式作用都不是必需的。
相似文献
1
Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.酿酒酵母HIS4基因的正向调控相互作用。
Mol Cell Biol. 1984 Jul;4(7):1326-33. doi: 10.1128/mcb.4.7.1326-1333.1984.
2
Multiple GCD genes required for repression of GCN4, a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae.酿酒酵母中抑制GCN4（氨基酸生物合成基因的转录激活因子）所需的多个GCD基因。
Mol Cell Biol. 1986 Nov;6(11):3990-8. doi: 10.1128/mcb.6.11.3990-3998.1986.
3
A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.酵母通用控制系统调控HIS4所必需的一段短核苷酸序列。
Cell. 1983 Jan;32(1):89-98. doi: 10.1016/0092-8674(83)90499-3.
4
Mutations in the structural genes for eukaryotic initiation factors 2 alpha and 2 beta of Saccharomyces cerevisiae disrupt translational control of GCN4 mRNA.酿酒酵母真核起始因子2α和2β的结构基因突变会破坏GCN4 mRNA的翻译控制。
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7515-9. doi: 10.1073/pnas.86.19.7515.
5
Functional analysis of the regulatory region adjacent to the cargB gene of Saccharomyces cerevisiae. Nucleotide sequence, gene fusion experiments and cis-dominant regulatory mutation analysis.
Eur J Biochem. 1987 Nov 16;169(1):193-200. doi: 10.1111/j.1432-1033.1987.tb13597.x.
6
Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.酿酒酵母LYS2和HIS4基因的Ty介导基因表达受相同的SPT基因控制。
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2431-4. doi: 10.1073/pnas.81.8.2431.
7
The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids.巴斯德毕赤酵母HIS4基因：核苷酸序列、非回复性his4缺失突变体的构建以及基于HIS4的复制型和整合型质粒的开发。
Curr Genet. 1994 Nov-Dec;26(5-6):443-50. doi: 10.1007/BF00309932.
8
RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene.酵母HIS4基因的BAS1/BAS2和GCN4依赖性转录需要RAP1。
Mol Cell Biol. 1991 Jul;11(7):3642-51. doi: 10.1128/mcb.11.7.3642-3651.1991.
9
Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.酿酒酵母中HIS4-lacZ融合基因的调控
Mol Cell Biol. 1982 Oct;2(10):1212-9. doi: 10.1128/mcb.2.10.1212-1219.1982.
10
Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae.Ty 插入对酿酒酵母中 HIS4 转录的影响。
Mol Cell Biol. 1984 Jul;4(7):1246-51. doi: 10.1128/mcb.4.7.1246-1251.1984.
引用本文的文献
1
Cytosolic and mitochondrial tRNA synthetase inhibitors increase lifespan in a /-dependent manner.胞质和线粒体氨酰-tRNA合成酶抑制剂以一种依赖于自噬的方式延长寿命。
iScience. 2022 Oct 21;25(11):105410. doi: 10.1016/j.isci.2022.105410. eCollection 2022 Nov 18.
2
Mutational analysis of the alpha subunit of eIF2B provides insights into the role of eIF2B bodies in translational control and VWM disease.真核起始因子 2B 阿尔法亚基突变分析为 eIF2B 体在翻译调控和 VWM 疾病中的作用提供了新见解。
J Biol Chem. 2021 Jan-Jun;296:100207. doi: 10.1074/jbc.RA120.014956. Epub 2021 Jan 30.
3
The Role of Transcription Factors and Nuclear Pore Proteins in Controlling the Spatial Organization of the Yeast Genome.转录因子和核孔蛋白在控制酵母基因组空间组织中的作用。
Dev Cell. 2019 Jun 17;49(6):936-947.e4. doi: 10.1016/j.devcel.2019.05.023.
4
The Histone Acetyltransferases Are Important for Morphogenesis, DON Biosynthesis, and Pathogenicity.组蛋白乙酰转移酶对形态发生、脱氧雪腐镰刀菌烯醇生物合成和致病性很重要。
Front Microbiol. 2018 Apr 26;9:654. doi: 10.3389/fmicb.2018.00654. eCollection 2018.
5
The sensitivity of the yeast, , to acetic acid is influenced by and .酵母对醋酸的敏感性受[具体因素1]和[具体因素2]的影响。 （由于原文中“ ”部分内容缺失，所以翻译中用[具体因素1]和[具体因素2]替代）
PeerJ. 2017 Nov 14;5:e4037. doi: 10.7717/peerj.4037. eCollection 2017.
6
Efficient prediction of human protein-protein interactions at a global scale.在全球范围内高效预测人类蛋白质-蛋白质相互作用。
BMC Bioinformatics. 2014 Dec 10;15(1):383. doi: 10.1186/s12859-014-0383-1.
7
Genetic basis for Saccharomyces cerevisiae biofilm in liquid medium.酿酒酵母在液体培养基中生物膜形成的遗传基础。
G3 (Bethesda). 2014 Jul 9;4(9):1671-80. doi: 10.1534/g3.114.010892.
8
The p38-interacting protein (p38IP) regulates G2/M progression by promoting α-tubulin acetylation via inhibiting ubiquitination-induced degradation of the acetyltransferase GCN5.p38 相互作用蛋白 (p38IP) 通过抑制乙酰转移酶 GCN5 的泛素化诱导降解来促进 α-微管蛋白乙酰化，从而调节 G2/M 期进程。
J Biol Chem. 2013 Dec 20;288(51):36648-61. doi: 10.1074/jbc.M113.486910. Epub 2013 Nov 12.
9
Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated.葡萄糖耗竭通过 eIF4A 的丢失和随后的 48S 起始前复合物的积累来抑制翻译起始，而磷酸戊糖途径则被协调地上调。
Mol Biol Cell. 2011 Sep;22(18):3379-93. doi: 10.1091/mbc.E11-02-0153. Epub 2011 Jul 27.
10
Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast.酵母中通过有丝分裂促进复合物突变揭示拮抗的 Gcn5-Hda1 相互作用。
Cell Div. 2011 Jun 8;6(1):13. doi: 10.1186/1747-1028-6-13.
本文引用的文献
1
Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae.酿酒酵母中氨基酸生物合成全局调控的生物学作用。
Mol Cell Biol. 1981 Jul;1(7):584-93. doi: 10.1128/mcb.1.7.584-593.1981.
2
The nucleotide sequence of the HIS4 region of yeast.酵母HIS4区域的核苷酸序列。
Gene. 1982 Apr;18(1):47-59. doi: 10.1016/0378-1119(82)90055-5.
3
Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.酿酒酵母中HIS4-lacZ融合基因的调控
Mol Cell Biol. 1982 Oct;2(10):1212-9. doi: 10.1128/mcb.2.10.1212-1219.1982.
4
Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.在大肠杆菌中与β-半乳糖苷酶融合的酵母基因能够在酵母中正常表达。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2460-4. doi: 10.1073/pnas.78.4.2460.
5
Regulatory sites for his3 gene expression in yeast.酵母中his3基因表达的调控位点。
Nature. 1982 Nov 18;300(5889):285-6. doi: 10.1038/300284a0.
6
Positive regulation in the general amino acid control of Saccharomyces cerevisiae.酿酒酵母一般氨基酸控制中的正调控
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374-8. doi: 10.1073/pnas.80.17.5374.
7
Participation of transcriptional and post-transcriptional regulatory mechanisms in the control of arginine metabolism in yeast.转录和转录后调控机制在酵母精氨酸代谢控制中的作用。
Mol Gen Genet. 1983;189(1):148-56. doi: 10.1007/BF00326068.
8
Identification of AAS genes and their regulatory role in general control of amino acid biosynthesis in yeast.酵母中氨基酸合成相关基因的鉴定及其在氨基酸生物合成总体调控中的作用。
Proc Natl Acad Sci U S A. 1983 May;80(9):2704-8. doi: 10.1073/pnas.80.9.2704.
9
A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.酵母通用控制系统调控HIS4所必需的一段短核苷酸序列。
Cell. 1983 Jan;32(1):89-98. doi: 10.1016/0092-8674(83)90499-3.
10
Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.酵母URA3基因的结构与功能。酵母中重叠编码序列的杂交β-半乳糖苷酶的差异调节表达。
J Mol Biol. 1983 Nov 15;170(4):883-904. doi: 10.1016/s0022-2836(83)80193-4.
Zotero 插件