Serrano-Alcalde Francisco, García-Aznar José Manuel, Gómez-Benito María José
Multiscale in Mechanical and Biological Engineering (M2BE), Aragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain.
Multiscale in Mechanical and Biological Engineering (M2BE), Aragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain.
J Theor Biol. 2017 Nov 7;432:25-32. doi: 10.1016/j.jtbi.2017.07.028. Epub 2017 Aug 9.
Despite the relevant regulatory role that nuclear deformation plays in cell behaviour, a thorough understanding of how fluid flow modulates the deformation of the cell nucleus in non-confined environments is lacking. In this work, we investigated the dynamics of cell deformation under different creeping flows as a general simulation tool for predicting nuclear stresses and strains. Using this solid-fluid modelling interaction framework, we assessed the stress and strain levels that the cell nucleus experiences as a function of different microenvironmental conditions, such as physical constraints, fluid flows, cytosol properties, and nucleus properties and size. Therefore, the simulation methodology proposed here allows the design of deformability-based experiments involving fluid flow, such as real-time deformability cytometry and dynamic cell culture in bioreactors or microfluidic devices.
尽管核变形在细胞行为中发挥着相关调节作用，但目前仍缺乏对流体流动如何在非受限环境中调节细胞核变形的全面理解。在这项工作中，我们研究了不同蠕动流作用下细胞变形的动力学，将其作为预测核应力和应变的通用模拟工具。利用这种固液建模相互作用框架，我们评估了细胞核在不同微环境条件（如物理约束、流体流动、细胞质特性以及细胞核特性和大小）作用下所经历的应力和应变水平。因此，这里提出的模拟方法允许设计基于变形性的涉及流体流动的实验，如实时变形性细胞术以及在生物反应器或微流控装置中的动态细胞培养。
